South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences; Key Lab of Aquatic Product Processing, Ministry of Agriculture and Rural Affairs of the People's Republic of China, Guangzhou 510300, P. R. China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, P.R. China.
J Microbiol Biotechnol. 2021 Jan 28;31(1):33-42. doi: 10.4014/jmb.2006.06002.
Due to the potential of antioxidants to scavenge free radicals in human body, it is important to be able to prepare antioxidant peptides that meet the industrial requirements for cosmetics and food. Here, we determined in vivo/in vitro activities of antioxidant peptide from (PFAOP) prepared by bio-fermentation method. The antioxidant property test results showed the DPPH, hydroxyl, superoxide radical-scavenging, and cellular antioxidant activity. EC values of PFAOPs were 0.018 ± 0.005, 0.126 ± 0.008, 0.168 ± 0.005, and 0.105 ± 0.005 mg/ml, respectively, exhibiting higher antioxidant activities than glutathione ( < 0.05). Moreover, anti-proliferation and cytotoxicity activity results illustrated PFAOP has a potent anti-proliferative activity against HepG2, Caco-2, and MCF-7 carcinoma cells with no cytotoxicity. Moreover, the protocols we developed in this work demonstrated several excellent advantages in PFAOP preparation compared to enzymatic hydrolysis or chemical synthesis methods and provide a theoretical foundation for higher-value application of marine-derived functional peptides.
由于抗氧化剂具有清除人体内自由基的潜力，因此能够制备出符合化妆品和食品工业要求的抗氧化肽非常重要。在这里，我们通过生物发酵法确定了（PFAOP）抗氧化肽的体内/体外活性。抗氧化性能测试结果表明，DPPH、羟基、超氧自由基清除和细胞抗氧化活性。PFAOP 的 EC 值分别为 0.018 ± 0.005、0.126 ± 0.008、0.168 ± 0.005 和 0.105 ± 0.005 mg/ml，表现出比谷胱甘肽（<0.05）更高的抗氧化活性。此外，抗增殖和细胞毒性活性结果表明，PFAOP 对 HepG2、Caco-2 和 MCF-7 癌细胞具有很强的抗增殖活性，且无细胞毒性。此外，与酶解或化学合成方法相比，我们在本工作中开发的方案在 PFAOP 制备方面具有几个优异的优势，并为海洋衍生功能肽的更高价值应用提供了理论基础。
