Pistor S, Hobom G
Institut für Mikrobiologie und Molekularbiologie, Justus-Liebig-Universität Giessen.
Klin Wochenschr. 1988 Feb 1;66(3):110-6. doi: 10.1007/BF01774224.
Expression of a foreign protein molecule on the E. coli bacterial surface has been achieved through hybrid plasmid construction of fusion proteins using outer membrane protein ompA as a carrier system. Influenza virus hemagglutinin fusion proteins of this character have been shown to become integrated into the bacterial outer membrane and to expose their hemagglutinin moiety at the exterior surface in a conformation which is at least similar to the authentic viral antigen structure.
通过构建以外膜蛋白ompA为载体系统的融合蛋白杂交质粒，已实现了在大肠杆菌细菌表面表达外源蛋白分子。已证明具有这种特性的流感病毒血凝素融合蛋白可整合到细菌外膜中，并以至少与真实病毒抗原结构相似的构象将其血凝素部分暴露于外表面。
