酿酒酵母甲酰四氢叶酸相互转化途径的突变体
Mutants of formyltetrahydrofolate interconversion pathway of Saccharomyces cerevisiae.
作者信息
McKenzie K Q, Jones E W
出版信息
Genetics. 1977 May;86(1):85-102. doi: 10.1093/genetics/86.1.85.
Abstract
Thirteen mutants of Saccharomyces cerevisiae that lack one or more of the three enzyme activities of the pathway for interconversion of tetrahydrofolate coenzymes at the formate level of oxidation have been isolated. They do not require adenine. All fail to complement mutations in the ade3 locus. Mutations that greatly reduce activity for the other two interconversion enzymes. The three enzyme activities cochromatograph on TEAE-cellulose columns. A mutation that eliminates synthetase activity also alters the chromatographic behavior of the remaining cyclohydrolase and dehydrogenase activities. It is suggested that the three activities reside in an enzyme complex encoded by the ade3 locus.
摘要
已分离出13种酿酒酵母突变体，它们在甲酸氧化水平上缺乏四氢叶酸辅酶相互转化途径的三种酶活性中的一种或多种。它们不需要腺嘌呤。所有突变体都不能互补ade3位点的突变。这些突变极大地降低了其他两种相互转化酶的活性。这三种酶活性在TEAE-纤维素柱上共色谱。消除合成酶活性的突变也会改变其余环水解酶和脱氢酶活性的色谱行为。有人提出，这三种活性存在于由ade3位点编码的酶复合物中。
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