Marin-Gómez Wendy, Grande Mᵃ José, Pérez-Pulido Rubén, Galvez Antonio, Lucas Rosario
Microbiology Division, Department of Health Sciences, Faculty of Experimental Sciences, University of Jaén, 23071 Jaén, Spain.
Foods. 2020 Aug 27;9(9):1184. doi: 10.3390/foods9091184.
Breast milk from a single mother was collected during a 28-week lactation period. Bacterial diversity was studied by amplicon sequencing analysis of the V3-V4 variable region of the 16S rRNA gene. and were the main phyla detected in the milk samples, followed by and . The proportion of to changed considerably depending on the sampling week. A total of 411 genera or higher taxons were detected in the set of samples. Genus was detected during the 28-week sampling period, at relative abundances between 2.0% and 68.8%, and it was the most abundant group in 14 of the samples. and had low relative abundances. At the genus level, bacterial diversity changed considerably at certain weeks within the studied period. The weeks or periods with lowest relative abundance of had more diverse bacterial compositions including genera belonging to that were poorly represented in the rest of the samples.
在一位母亲28周的哺乳期内收集了母乳。通过对16S rRNA基因V3 - V4可变区进行扩增子测序分析来研究细菌多样性。厚壁菌门和拟杆菌门是在乳汁样本中检测到的主要菌门，其次是放线菌门和变形菌门。厚壁菌门与拟杆菌门的比例根据采样周数有很大变化。在这组样本中总共检测到411个属或更高分类单元。在28周的采样期内均检测到了葡萄球菌属，其相对丰度在2.0%至68.8%之间，并且在14个样本中是最丰富的菌群。肠球菌属和链球菌属的相对丰度较低。在属水平上，在所研究时期内的某些周，细菌多样性有很大变化。葡萄球菌属相对丰度最低的周或时期具有更多样化的细菌组成，包括在其他样本中代表性较差的芽孢杆菌属。
