Arias Laís Salomão, Brown Jason L, Butcher Mark C, Delaney Christopher, Monteiro Douglas Roberto, Ramage Gordon
Department of Preventive and Restorative Dentistry, São Paulo State University (Unesp), School of Dentistry, Araçatuba, São Paulo, Brazil.
Oral Sciences Research Group, Glasgow Dental School, School of Medicine, Dentistry and Nursing, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
J Oral Microbiol. 2020 Jun 7;12(1):1771071. doi: 10.1080/20002297.2020.1771071.
Novel and new therapeutic strategies capable of enhancing the efficacy of existing antimicrobials is an attractive proposition to meet the needs of society.
This study aimed to evaluate the potentiating effect of a miconazole (MCZ) nanocarrier system, incorporated with iron oxide nanoparticles (IONPs) and chitosan (CS) (IONPs-CS-MCZ). This was tested on three representative complex interkingdom oral biofilm models (caries, denture and gingivitis).
The planktonic and sessile minimum inhibitory concentrations (MICs) of IONPs-CS-MCZ against different strains were determined, as well as against all represented bacterial species that formed within the three biofilm models. Biofilms were treated for 24 hours with the IONPs-CS nanocarrier system containing MCZ at 64 mg/L, and characterized using a range of bioassays for quantitative and qualitative assessment.
MIC results generally showed that IONPs-CS-MCZ was more effective than MCZ alone. IONPs-CS-MCZ also promoted reductions in the number of CFUs, biomass and metabolic activity of the representative biofilms, as well as altering biofilm ultrastructure when compared to untreated biofilms. IONPs-CS-MCZ affected the composition and reduced the CFEs for most of the microorganisms present in the three evaluated biofilms. In particular, the proportion of streptococci in the biofilm composition were reduced in all three models, whilst spp. percentage reduced in the gingivitis and caries models, respectively.
In conclusion, the IONPs-CS-MCZ nanocarrier was efficient against three models of pathogenic oral biofilms, showing potential to possibly interfere in the synergistic interactions among fungal and bacterial cells within polymicrobial consortia.
能够提高现有抗菌药物疗效的新型治疗策略是满足社会需求的一个有吸引力的提议。
本研究旨在评估一种与氧化铁纳米颗粒（IONPs）和壳聚糖（CS）结合的咪康唑（MCZ）纳米载体系统（IONPs-CS-MCZ）的增效作用。在三种具有代表性的复杂跨王国口腔生物膜模型（龋齿、义齿和牙龈炎）上进行了测试。
测定了IONPs-CS-MCZ对不同菌株的浮游和固着最低抑菌浓度（MIC），以及对三种生物膜模型中形成的所有代表性细菌种类的MIC。用含64mg/L MCZ的IONPs-CS纳米载体系统处理生物膜24小时，并使用一系列生物测定法进行定量和定性评估。
MIC结果总体显示，IONPs-CS-MCZ比单独的MCZ更有效。与未处理的生物膜相比，IONPs-CS-MCZ还促进了代表性生物膜的菌落形成单位数量、生物量和代谢活性的减少，并改变了生物膜的超微结构。IONPs-CS-MCZ影响了三种评估生物膜中大多数微生物的组成并降低了CFE。特别是，在所有三种模型中，生物膜组成中链球菌的比例均降低，而在牙龈炎和龋齿模型中， spp.的百分比分别降低。
总之，IONPs-CS-MCZ纳米载体对三种致病性口腔生物膜模型有效，显示出可能干扰多微生物群落中真菌和细菌细胞之间协同相互作用的潜力。
