Ababneh Mustafa, Hananeh Wael, Alzghoul Mohammad
Department of Basic Medical Veterinary Sciences, Jordan University of Science & Technology, P.O. Box 3030, Irbid, 22110, Jordan.
Department of Pathology and Public Health, Jordan University of Science & Technology, P.O. Box 3030, Irbid, 22110, Jordan.
Heliyon. 2020 Aug 31;6(8):e04827. doi: 10.1016/j.heliyon.2020.e04827. eCollection 2020 Aug.
Koi herpesvirus disease is a serious disease affecting both wild and common carp species in different continents throughout the world. Based on pathological and molecular findings, we document the presence of koi herpesvirus disease in Iraq as a cause of mass mortality among the common carp of the Tigris river. On a macroscopic level, the fish exhibited variably sized skin ulcerations throughout the entire trunk. The gills showed variable degrees of discoloration with an increased amount of slimy mucus. Microscopically, degeneration and necrosis with infiltration of a heterogenous population of inflammatory cells characterized different organs, primarily the skin and gills, with occasional intranuclear inclusion bodies that are consistent with koi herpesvirus disease. A semi-nested PCR assay coupled with sequencing confirmed the pathological diagnosis. Genotyping and sequence analysis of the TK gene, ORF 136 and markers I and II identified the isolated CyHV-3 as variant A1 of the Asian genotype TUSMT1 (J strain) displaying the III allele.
锦鲤疱疹病毒病是一种严重疾病，影响着世界不同大洲的野生鲤鱼和普通鲤鱼品种。基于病理学和分子学研究结果，我们记录了伊拉克存在锦鲤疱疹病毒病，它是底格里斯河普通鲤鱼大量死亡的原因。在宏观层面，鱼的整个躯干出现大小不一的皮肤溃疡。鳃呈现不同程度的变色，黏液增多。在微观层面，不同器官（主要是皮肤和鳃）出现变性和坏死，并伴有异质性炎症细胞浸润，偶尔可见与锦鲤疱疹病毒病相符的核内包涵体。半巢式聚合酶链反应（PCR）检测及测序证实了病理学诊断。对胸苷激酶（TK）基因、开放阅读框136以及标记I和标记II进行基因分型和序列分析，确定分离出的鲤疱疹病毒3型（CyHV-3）为亚洲基因型TUSMT1（J株）的A1变体，表现为III等位基因。
