Saadat Saman, Karami Pezhman, Jafari Mohammad, Kholoujini Mahdi, Rikhtegaran Tehrani Zahra, Mohammadi Younes, Alikhani Mohammad Yousef
Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Shahid Fahmideh Street, Hamadan, Iran, 65178.
Department of Microbiology, Sarem Cell Research Center, Sarem Women's Hospital, Phase 3 Ekbatan, Tehran, Iran, 13969-56111.
Pathog Dis. 2020 Oct 7;78(7). doi: 10.1093/femspd/ftaa037.
Mycoplasma hominis, an opportunistic pathogen in human genitourinary tract, can cause chronic infection in the prostate. Intracellular survival of M. hominis leads to a prolonged presence in the host cells that can affect the cell's biological cycle. In the present study, we aimed to evaluate the frequency of M. hominis DNA in prostate tissue of Iranian patients with prostate cancer (PCa) in comparison to a control group with benign prostatic hyperplasia (BPH).
This research was a retrospective case-control study using 61 archived formalin-fixed paraffin-embedded (FFPE) blocks of prostate tissue from patients with PCa and 70 FFPE blocks of patients with BPH. Real-time PCR, targeting two different genes, 16S rRNA and yidC, in the M. hominis genome was performed for all specimens.
Out of 61 blocks of prostate biopsy from patients with PCa, eight samples (13%) were positive for M. hominis, while the bacterium was not detected in any of the 70 blocks of patients with BPH (P value, 0.002).
The high frequency of M. hominis in patients with PCa likely shows a hidden role of the organism in prostate cancer during its chronic, apparently silent and asymptomatic colonization in prostate.
人型支原体是人类泌尿生殖道的一种机会致病菌，可导致前列腺慢性感染。人型支原体在细胞内存活会导致其在宿主细胞中长时间存在，进而影响细胞的生物周期。在本研究中，我们旨在评估伊朗前列腺癌（PCa）患者前列腺组织中人型支原体DNA的频率，并与良性前列腺增生（BPH）对照组进行比较。
本研究为回顾性病例对照研究，使用了61例前列腺癌患者的存档福尔马林固定石蜡包埋（FFPE）前列腺组织块和70例BPH患者的FFPE组织块。对所有标本进行实时PCR，靶向人型支原体基因组中的两个不同基因，即16S rRNA和yidC。
在61例前列腺癌患者的前列腺活检组织块中，8个样本（13%）人型支原体呈阳性，而70例BPH患者的组织块中均未检测到该细菌（P值为0.002）。
PCa患者中人型支原体的高频率可能表明该生物体在前列腺癌中存在隐藏作用，即在前列腺中进行慢性、明显隐匿且无症状的定植过程中发挥作用。
