Narimani Ramin, Azizi Mehdi, Esmaeili Mahdad, Rasta Seyed Hossein, Khosroshahi Hamid Tayebi
Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Medical Bioengineering Department, School of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
3 Biotech. 2020 Oct;10(10):416. doi: 10.1007/s13205-020-02405-z. Epub 2020 Sep 3.
Creatinine concentration is one of the important elements in the body for diagnosing kidney failure, muscular dystrophy, glomerular filtration rate, and diabetic nephropathy. The disadvantages of recently introduced analytical techniques, such as Jaffe's, spectroscopic, colorimetric, and chromatographic methods, for quantifying creatinine in urine involve toxicity, the high cost, interference, and the complexity of the design. In this paper, we designed and fabricated a new colorimetric assay for the measurement of creatinine concentration based on color differentiation generated by mixing different concentrations of creatinine with synthesized silver nanoparticles (AgNPs) coated with polyvinylpyrrolidone (PVP) and polyvinyl alcohol (PVA). An isolated box is designed for the uniform optical imaging of solutions, the captured images are processed in real time, and the quantitative and qualitative results are displayed. For colorimetric processing, a variety of color systems, such as RGB (red, green, blue), CMYK (cyan, magenta, yellow, black), and grayscale (Gr), have been evaluated, indicating that the combination of green (G) and grayscale (Gr) provides the best results for this experiment. TEM analysis and spectroscopy were used to confirm the results of the experiment. Linear range and limit of detection (LOD) were obtained for AgNPs/PVP 0.03-1 mg/dl and 0.024 mg/dl and for AgNPs/PVA 0.01-1 mg/dl and 0.014 mg/dl, respectively, indicating the superiority of our proposed method over recently introduced methods. In this experiment, the detectable resolution with AgNPs/PVP is 40, while it is 71 with AgNPs/PVA. The designed system is simple to use, small in size, and cost-effective for measuring creatinine concentration, while it can be used as a portable system.
肌酐浓度是诊断肾衰竭、肌肉萎缩症、肾小球滤过率和糖尿病肾病的重要指标之一。最近引入的用于定量尿液中肌酐的分析技术，如杰氏法、光谱法、比色法和色谱法，存在毒性、成本高、干扰以及设计复杂等缺点。在本文中，我们设计并制造了一种新的比色测定法，用于测量肌酐浓度，该方法基于将不同浓度的肌酐与包覆有聚乙烯吡咯烷酮（PVP）和聚乙烯醇（PVA）的合成银纳米颗粒（AgNPs）混合产生的颜色差异。设计了一个隔离箱用于溶液的均匀光学成像，实时处理捕获的图像，并显示定量和定性结果。对于比色处理，评估了多种颜色系统，如RGB（红、绿、蓝）、CMYK（青、品红、黄、黑）和灰度（Gr），结果表明绿色（G）和灰度（Gr）的组合为本实验提供了最佳结果。使用透射电子显微镜（TEM）分析和光谱学来确认实验结果。AgNPs/PVP的线性范围和检测限（LOD）分别为0.03 - 1mg/dl和0.024mg/dl，AgNPs/PVA的线性范围和检测限分别为0.01 - 1mg/dl和0.014mg/dl，这表明我们提出的方法优于最近引入的方法。在本实验中，AgNPs/PVP的可检测分辨率为40，而AgNPs/PVA的可检测分辨率为71。所设计的系统使用简单、体积小且测量肌酐浓度具有成本效益，同时可作为便携式系统使用。
