Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin, China.
Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Fitoterapia. 2020 Oct;146:104726. doi: 10.1016/j.fitote.2020.104726. Epub 2020 Sep 16.
Four new indole alkaloids, daturametelindoles A-D (1-4) were isolated from the EtOAc soluble partition of the ethanol extract of the Datura metel seeds. The structures of the new compounds were determined based on spectroscopic evidence, including their 1D- and 2D-NMR spectra and mass spectrometry. In particular, compounds 1-4 were all racemes, confirmed by the optical rotations and CD spectra. Unfortunately, the chiral monomers were not obtained due to the amount, but the developments of their chiral separation and chiral resolution were completed. All isolated compounds were evaluated for cytotoxic effects against human gastric adenocarcinoma cells (SGC-7901), human hepatoma (Hepg2), and human breast cancer (MCF-7).
从曼陀罗种子的乙醇提取物的 EtOAc 可溶部分分离得到四个新的吲哚生物碱，即颠茄麦特灵 A-D（1-4）。根据包括 1D-和 2D-NMR 光谱和质谱在内的光谱证据确定了新化合物的结构。特别是，化合物 1-4 均为外消旋体，通过旋光度和 CD 光谱证实。不幸的是，由于量的原因，没有获得手性单体，但完成了它们的手性分离和手性拆分的发展。所有分离得到的化合物都针对人胃癌细胞（SGC-7901）、人肝癌（HepG2）和人乳腺癌（MCF-7）进行了细胞毒性评估。
