Division of Biochemistry, Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
Division of Microbiology, Department of Basic Sciences, School of Medicine, Loma Linda University, Loma Linda, CA, USA.
Mol Oral Microbiol. 2020 Dec;35(6):251-259. doi: 10.1111/omi.12314. Epub 2020 Oct 10.
Porphyromonas gingivalis is a gram-negative anaerobic bacterium and an etiologic agent of adult periodontitis. By inducing a dysbiotic state within the host microbiota it contributes to a chronic inflammatory environment in the oral cavity. Under some circumstances, the oral bacteria may gain access to systemic circulation. While the most widely recognized function of platelets is to reduce hemorrhage in case of vascular damage, it is known that platelets are also involved in the hematologic responses to bacterial infections. Some pathogenic bacteria can interact with platelets, triggering their activation and aggregation. The aim of this study was to assess platelet responses to the presence of P. gingivalis in whole blood. Human whole blood was pretreated with P. gingivalis and then platelet plug formation was measured under high shear conditions using the PFA-100. In the presence of P. gingivalis, time for a platelet plug to occlude the aperture in the collagen/ADP cartridge was shortened in a manner dependent on bacterial concentration and the duration of bacterial preincubation of blood. P. gingivalis enhances thrombus forming potential of platelets in whole blood.
牙龈卟啉单胞菌是一种革兰氏阴性厌氧菌，也是成人牙周炎的病原体。它通过在宿主微生物群中诱导一种生态失调状态，导致口腔内出现慢性炎症环境。在某些情况下，口腔细菌可能会进入全身循环。虽然血小板最广泛的功能是在血管损伤时减少出血，但已知血小板也参与了对细菌感染的血液反应。一些致病菌可以与血小板相互作用，触发它们的激活和聚集。本研究旨在评估血小板对全血中牙龈卟啉单胞菌存在的反应。用人全血预先处理牙龈卟啉单胞菌，然后在高剪切条件下使用 PFA-100 测量血小板塞的形成。在牙龈卟啉单胞菌存在的情况下，血小板塞阻塞胶原/ADP 小室孔的时间随着细菌浓度和血液中细菌预孵育时间的延长而缩短。牙龈卟啉单胞菌增强了全血中血小板形成血栓的潜力。
