Departamento de Virologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife, Pernambuco, Brasil.
Laboratório de Virologia Comparativa e Ambiental, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
Braz J Med Biol Res. 2020 Sep 18;53(11):e9529. doi: 10.1590/1414-431X20209529. eCollection 2020.
Norovirus (NoV) is the main cause of gastroenteritis outbreaks worldwide. Although NoV spreads mainly from person to person, it is estimated that a large proportion of NoV outbreaks are caused by foodborne transmission. Bivalve mollusks are one of the most important foods involved in NoV transmission to humans. Little is known about NoV prevalence in shellfish harvested and commercialized in Brazil. The aim of this study was to map, for the first time, the distribution of NoV contamination in oysters and mussels harvested and commercialized in the coast of Pernambuco state, northeast Brazil. A total of 380 mollusks (260 oysters and 120 mussels) were collected between February and August 2017 either directly from harvesting areas or obtained from beach vendors at 17 sites in Pernambuco. Samples were processed and tested for NoV contamination using a SYBR Green real-time PCR assay. All samples were negative for NoV GI or GII contamination, suggesting a low risk of NoV contamination from this food source during the study period. Additional surveys in different areas of the Brazilian coast are warranted to monitor the risk of NoV infection upon seafood consumption.
诺如病毒（NoV）是全球范围内引起肠胃炎暴发的主要原因。虽然 NoV 主要通过人与人之间传播，但据估计，很大一部分 NoV 暴发是由食物传播引起的。双壳贝类是与人类 NoV 传播有关的最重要食物之一。在巴西收获和商业化的贝类中，NoV 的流行情况知之甚少。本研究的目的是首次绘制巴西东北部伯南布哥州沿海地区收获和商业化的牡蛎和贻贝中 NoV 污染的分布情况。2017 年 2 月至 8 月间，在伯南布哥州的 17 个地点，从收获区直接或从海滩商贩处共采集了 380 个贝类（260 个牡蛎和 120 个贻贝）。使用 SYBR Green 实时 PCR 检测法对样品进行了 NoV 污染检测。所有样品均未检出 NoV GI 或 GII 污染，表明在研究期间，该食物源的 NoV 污染风险较低。需要在巴西沿海的不同地区进行额外的调查，以监测食用海鲜时 NoV 感染的风险。
