Péter O, Dupuis G, Peacock M G, Burgdorfer W
J Clin Microbiol. 1987 Jun;25(6):1063-7. doi: 10.1128/jcm.25.6.1063-1067.1987.
An enzyme-linked immunosorbent assay (ELISA) was developed to detect immunoglobulin G to Coxiella burnetii phase II. Serum samples from 213 patients who had had Q fever 1 year previously and from 301 blood donors from six localities in Switzerland were tested by ELISA and by indirect fluorescent-antibody (IFA) and complement fixation (CF) tests. The ELISA and the IFA and CF tests detected antibody to C. burnetii in 202 (94.8%), 193 (90.6%), and 166 (77.8%) of the 213 Q fever patients, respectively. With the serum samples from blood donors, the ELISA yielded a higher percentage of positive sera than did the IFA and CF tests. The high specificity of the three tests was confirmed by analyzing paired serum samples from 36 patients suffering from acute pneumonia of viral or bacterial origin. In these cases, the serological results were negative by the three tests, except for three Q-fever cases included as positive control.
开发了一种酶联免疫吸附测定(ELISA)来检测针对伯纳特柯克斯体II相的免疫球蛋白G。通过ELISA以及间接荧光抗体(IFA)和补体结合(CF)试验,对213例1年前患Q热的患者的血清样本以及来自瑞士六个地区的301名献血者的血清样本进行了检测。ELISA、IFA和CF试验分别在213例Q热患者中的202例(94.8%)、193例(90.6%)和166例(77.8%)中检测到了针对伯纳特柯克斯体的抗体。对于献血者的血清样本,ELISA检测出阳性血清的比例高于IFA和CF试验。通过分析36例患有病毒或细菌源性急性肺炎患者的配对血清样本,证实了这三种试验具有高特异性。在这些病例中,除了作为阳性对照纳入的3例Q热病例外,这三种试验的血清学结果均为阴性。