Aqueous extract of fruit pulp exhibits antihyperglycaemic activity.

OBJECTIVE

Linn. () is a well-known plant used in traditional medicine. The plant is popular for its antidiabetic activity. However, effect so f its aqueous fruit pulp extract on carbohydrate hydrolyzing enzymes and its glucose uptake potential were not explored.

MATERIALS AND METHODS

The antidiabetic activity was assessed by α-amylase and α-glucosidase inhibitory assays after preliminary phytochemical analysis. MTT assay was carried out to find cytotoxicity. Glucose uptake activity of the extract was carried out using L6 myotubes.

RESULTS

The results showed a strong α-amylase inhibitory activity for the fruit pulp extract of compared to standard acarbose; the IC of the fruit pulp extract of and acarbose was 34.19 µg/ml 34.83µM. The extract also showed moderate α-glucosidase inhibitory activity. IC of the fruit pulp extract of and acarbose were 56.91µg/ml and 45.69µM respectively. The cytotoxicity assay showed IC of >300µg/ml and ≥1000µM for the fruit pulp extract of and metformin. The extract showed 63.99±0.08% glucose uptake in L6 myotubes whereas metformin and insulin at 10µg/ml and 10µM exhibited an uptake of 76.99±0.3% and 84.48±0.45% glucose, respectively.

CONCLUSION

The study revealed that the fruit pulp extract of Linn does not show any cytotoxic effect and has very good α-amylase and good α-glucosidase inhibitory activities. The glucose uptake potential proves its postprandial hypoglycemic effect. Hence, it may be considered an antidiabetic agent for control of postprandial hyperglycemia.