Antiglycation and antitumoral activity of dry extract.

OBJECTIVE

Investigation of the antiglycation and antitumoral potential of standardized and saponins-enriched extracts of herbal medicine.

MATERIALS AND METHODS

The procedures for the evaluation of the antiglycation activity of the standardized (TtSE) and saponins-enriched (TtEE) extracts of were: determination of relative mobility in electrophoresis (RME), free amino groups using OPA method and advanced glycation end-products (AGEs) fluorescence. Antioxidant activity was determined by DPPH radical scavenging test. antitumor activity of TtSE and TtEE was evaluated in human tumor cell lines.

RESULTS

The results were obtained by antiglycation tests (RME, OPA method and AGEs fluorescence determination), using BSA as protein and ribose as glycation agent, and antioxidant assay (DPPH test); it was verified that both extracts of have antiglycation and antioxidant activity. In addition, the extracts were able to induce death of more than 50% of human tumor cell lines.

CONCLUSION

The present study showed that standardized and saponins-enriched extracts of herbal medicine present antiglycation and antioxidant and antiproliferative action in human tumor cells lines. The saponins-enriched extract proved a greater antiglycation and antioxidant activity in comparison to the standardized type.