Department of Healthcare Biotechnology, Atta-ur-Rahman School of Applied Biosciences, National University of Sciences and Technology, Islamabad, Pakistan.
Department of Histopathology, Armed Forces Institute of Pathology, Rawalpindi, Pakistan.
Mol Biol Rep. 2020 Oct;47(10):7861-7870. doi: 10.1007/s11033-020-05864-y. Epub 2020 Oct 4.
Methylphenidate (MPH), a psychotropic medication is commonly used for children with attention deficit hyperactivity disorder (ADHD). In this study we elucidated the neuroprotective and anti-inflammatory effects of MPH and Rosmarinus officinalis (rosemary) extract, an ancient aromatic herb with several applications in traditional medicine. Briefly, six groups of mice (n = 8 each group), were specified for the study and behavioral analysis was performed to analyze spatial memory followed by histological assessment and gene expression analysis of synaptic (Syn I, II and III) and inflammatory markers (IL-6, TNFα and GFAP) via qRT-PCR, in an AlCl-induced mouse model for neurotoxicity. The behavioral analysis demonstrated significant cognitive decline, memory defects and altered gene expression in AlCl-treated group. Rosemary extract significantly decreased the expression of inflammatory and synaptic markers to the similar levels as that of MPH. The present findings suggested the neuroprotective potential of Rosmarinus officinalis extract. However, further characterization of its anti-inflammatory and neuroprotective properties and MPH is required to strategize future treatments for several neurological and neurodegenerative disorders, including Alzheimer's disease.
哌醋甲酯（MPH）是一种精神药物，常用于治疗注意力缺陷多动障碍（ADHD）的儿童。在这项研究中，我们阐明了 MPH 和迷迭香（迷迭香）提取物的神经保护和抗炎作用，迷迭香是一种古老的芳香草本植物，在传统医学中有多种应用。简要地说，将六组小鼠（每组 n=8）用于研究，并通过 qRT-PCR 进行空间记忆的行为分析，随后进行组织学评估和突触（Syn I、II 和 III）和炎症标志物（IL-6、TNFα 和 GFAP）的基因表达分析，在 AlCl 诱导的神经毒性小鼠模型中。行为分析表明，AlCl 处理组的认知能力显著下降，记忆力缺陷，基因表达发生改变。迷迭香提取物可显著降低炎症和突触标志物的表达，使其水平与 MPH 相似。目前的研究结果表明迷迭香提取物具有神经保护潜力。然而，需要进一步表征其抗炎和神经保护特性以及 MPH，以为包括阿尔茨海默病在内的几种神经和神经退行性疾病制定未来的治疗策略。
