Kim Si Chul, Chung Sang Ok, Lee Hyo Jung
Department of Biology, Kunsan National University, 558 Daehak-ro, Gunsan-si, 54150, Republic of Korea.
Tidal Flat Research Center-National Institute of Fisheries Science, Gunsan, Republic of Korea.
Curr Microbiol. 2021 Jan;78(1):411-416. doi: 10.1007/s00284-020-02231-9. Epub 2020 Oct 8.
A novel strain KS-M25 was isolated from estuary water in South Korea. Strain KS-M25 was Gram-staining-negative, strictly aerobic, motile rods-shaped bacterium and showed oxidase- and catalase-positive reactions. Growth of strain KS-M25 was observed at 10-25 °C (optimum, 20 °C), at pH 5.5-9.0 (optimum, pH 7.5), and with 0-6.0% (w/v) NaCl (optimum, 1%). Ubiquinone-8 was identified as the sole isoprenoid quinone and the major fatty acids were C cyclo-C and sum in feature 3 (comprising C ω7c and/or C ω6c). The G+C content values based on genome sequences was 62.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain KS-M25 formed a clear cluster within the genus Achromobacter. Strain KS-M25 was most closely related to Achromobacter anxifer LMG 26857, Achromobacter dolens LMG 26840, and Achromobacter xylosoxidans NBRC 15126 with 97.8%, 97.8%, and 97.7% 16S rRNA gene sequence similarities, respectively. Based on phenotypic, chemotaxonomic, and phylogenetic analysis, strain KS-M25 represents a novel species of the genes Achromobacter, for which the name Achromobacter aestuarii sp. nov. is proposed. The type strain is KS-M25 (= KACC 21219 = JCM 33329).
从韩国河口水中分离出一株新型菌株KS-M25。菌株KS-M25革兰氏染色阴性，严格需氧，为运动性杆状细菌，氧化酶和过氧化氢酶反应呈阳性。菌株KS-M25在10-25℃（最适温度为20℃）、pH 5.5-9.0（最适pH为7.5）以及含0-6.0%（w/v）NaCl（最适为1%）的条件下生长。泛醌-8被鉴定为唯一的类异戊二烯醌，主要脂肪酸为特征3中的环丙烷脂肪酸（包括C ω7c和/或C ω6c）。基于基因组序列的G+C含量值为62.3 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株KS-M25在无色杆菌属内形成了一个清晰的聚类。菌株KS-M25与无色杆菌属的Achromobacter anxifer LMG 26857、Achromobacter dolens LMG 26840和木糖氧化无色杆菌NBRC 15126关系最为密切，其16S rRNA基因序列相似性分别为97.8%、97.8%和97.7%。基于表型、化学分类和系统发育分析，菌株KS-M25代表无色杆菌属的一个新物种，为此提出名为河口无色杆菌（Achromobacter aestuarii sp. nov.）。模式菌株为KS-M25（=KACC 21219=JCM 33329）。
