Department of Biology, Bahir Dar University, Bahir Dar, Amhara Regional State, Ethiopia.
Department of Microbial Cellular and Molecular Biology, Addis Ababa University, Addis Ababa, Ethiopia.
PLoS One. 2020 Oct 9;15(10):e0239775. doi: 10.1371/journal.pone.0239775. eCollection 2020.
In this study, we evaluated the antibacterial activities of the essential oils (EOs) of Thymus serrulatus and Thymus schimperi collected from Ofla (Ofl), Alamata (Ala), Yilmana Densa (Yil), Tarmaer (Tar), Butajira (Buta), and Bale (Bal) in Ethiopia against cariogenic bacteria (Streptococcus mutans and Lactobacillus) isolated from human teeth. Inhibition zones (IZs), minimum inhibitory concentrations (MICs), and minimum bactericidal concentrations (MBCs) were measures of the antibacterial activity. Significant bacterial inhibitions resulted in a dose-and EO-dependent manner. At 128 μl/mL, IZs against S. mutans were 37.33 mm (Tar), 36.00 mm (Bal), 33.67 mm (Yil), 33.33 mm (Ofl), 30.00 (Ala), and 29.67 mm (Buta) and IZs against Lactobacillus were 31.00 mm (Tar), 30.67 mm (Yil), 27.67 (Bal), 27.00 (Buta), 26.67 (Ofl), and 21.33 (Ala). The respective inhibition zones due to 3% DMSO (negative control) and 3% H2O2 (positive control) were 0.00 mm/30.00 mm against S. mutans and 0.00 mm/29.00 mm against Lactobacillus. At 128 μl/mL dose, all the EOs resulted in significantly higher inhibition zones than that of 3% H2O2 against S. mutans and Lactobacillus.
在这项研究中，我们评估了来自奥弗拉（Ofla）、阿拉马塔（Alamata）、伊尔曼达登萨（Yilmana Densa）、塔尔马厄（Tarmaer）、布塔吉拉（Butajira）和巴莱（Bale）的奥弗拉（Ofla）、阿拉马塔（Alamata）、伊尔曼达登萨（Yilmana Densa）、塔尔马厄（Tarmaer）、布塔吉拉（Butajira）和巴莱（Bale）收集的绒毛百里香（Thymus serrulatus）和香薄荷（Thymus schimperi）精油（EOs）对从人牙中分离出的致龋细菌（变形链球菌和乳酸杆菌）的抗菌活性。抑菌圈（IZs）、最小抑菌浓度（MICs）和最小杀菌浓度（MBCs）是抗菌活性的衡量标准。细菌抑制的显著结果是剂量和精油依赖性的。在 128 μl/mL 时，对 S. mutans 的 IZs 分别为 37.33 mm（Tar）、36.00 mm（Bal）、33.67 mm（Yil）、33.33 mm（Ofl）、30.00 mm（Ala）和 29.67 mm（Buta），对 Lactobacillus 的 IZs 分别为 31.00 mm（Tar）、30.67 mm（Yil）、27.67 mm（Bal）、27.00 mm（Buta）、26.67 mm（Ofl）和 21.33 mm（Ala）。3% DMSO（阴性对照）和 3% H2O2（阳性对照）分别导致 0.00 mm/30.00 mm 对 S. mutans 和 0.00 mm/29.00 mm 对 Lactobacillus 的抑制带。在 128 μl/mL 剂量下，所有精油对 S. mutans 和 Lactobacillus 的抑制带均显著高于 3% H2O2。
