Myerowitz R L, Albers A C, Yee R B, Orskov F
J Clin Microbiol. 1977 Aug;6(2):124-7. doi: 10.1128/jcm.6.2.124-127.1977.
Two hundred and ninety-four isolates of Escherichia coli, including 105 from blood cultures, 94 from stools of hospital inpatients, and 96 from rectal cultures of healthy young adults, were biotyped by using the API-20E system and tested for the presence of K1 antigen. The overall frequency of K1 strains was 14.2% and was similar among the three sources. Forty-eight biotypes were observed, but two-thirds of all isolates, including two-thirds of the K1 strains, belonged to only five biotypes. Among the five commonest biotypes, the distribution of K1 strains was nonrandom, since 23 of the 27 K1 strains belonged to only two biotypes. Analysis of the O and H antigens of K1 strains indicated that this correlation of biotype with K1 antigen was due to a restricted number of serovars ("clones") that were repeatedly isolated from the population studied. These serovas included O18:K1:H7, O1:121:H6 and O16:K1:H6. Although a statistically significant correlation between biotype and K1 antigen was observed, the correlation was not sufficiently great to alow biotyping to be of significant predictive value as a marker for the K1 antigen.
采用API-20E系统对294株大肠杆菌进行了生物分型，并检测了K1抗原的存在情况。这些菌株包括105株来自血培养、94株来自医院住院患者的粪便以及96株来自健康年轻成年人的直肠培养物。K1菌株的总体频率为14.2%，在这三个来源中相似。观察到48种生物型，但所有分离株的三分之二，包括三分之二的K1菌株，仅属于五种生物型。在最常见的五种生物型中，K1菌株的分布并非随机，因为27株K1菌株中有23株仅属于两种生物型。对K1菌株的O抗原和H抗原分析表明，生物型与K1抗原的这种相关性是由于从所研究人群中反复分离出的血清型（“克隆”）数量有限所致。这些血清型包括O18:K1:H7、O1:121:H6和O16:K1:H6。虽然观察到生物型与K1抗原之间存在统计学上的显著相关性，但这种相关性还不够大，不足以使生物分型作为K1抗原的标志物具有显著的预测价值。
