Department of Oncology Taihe Hospital, Hubei University of Medicine, 32 South Renmin Road, Shiyan, Hubei, 442000, People's Republic of China.
Department of Radiology, Renmin Hospital, Hubei University of Medicine, Shiyan, Hubei, 442000, People's Republic of China.
BMC Pulm Med. 2020 Oct 20;20(1):273. doi: 10.1186/s12890-020-01281-w.
LncRNA SNHG10 has been reported to be an oncogenic lncRNA in liver cancer. However, its roles in non-small cell lung cancer (NSCLC) remains unknown.
Tumor and paired non-tumor tissues were harvested from 62 NSCLC patients. RT-qPCR was used to detect the expression of SNHG10 and miR-21 in tissues. Overexpression experiments were used to evaluate the interaction between SNHG10 and miR-21 in NSCLC cells. CCK-8 assay was used to detect the cell proliferation.
We observed the expression of SNHG10 was down-regulated in non-small cell lung cancer (NSCLC) compared with that in non-tumor tissues. Moreover, we found that high expression levels of SNHG10 predicted favorable survival of NSCLC patients, and the expression of miR-21 were increased in NSCLC and inversely correlated with SNHG10 expression. In NSCLC cells, overexpression of SNHG10 resulted in increased miR-21 gene methylation and decreased miR-21 expression. Moreover, overexpression of SNHG10 attenuated the enhancing effect of miR-21 overexpression on cell proliferation.
SNHG10 may involve in NSCLC cell proliferation by regulating the miR-21 gene methylation.
长链非编码 RNA SNHG10 已被报道在肝癌中是一种致癌的 lncRNA。然而，其在非小细胞肺癌（NSCLC）中的作用尚不清楚。
从 62 名 NSCLC 患者中采集肿瘤和配对的非肿瘤组织。使用 RT-qPCR 检测组织中 SNHG10 和 miR-21 的表达。过表达实验用于评估 SNHG10 和 miR-21 在 NSCLC 细胞中的相互作用。CCK-8 测定法用于检测细胞增殖。
我们观察到 SNHG10 在非小细胞肺癌（NSCLC）中的表达低于非肿瘤组织。此外，我们发现 SNHG10 的高表达水平预示着 NSCLC 患者的良好预后，并且 miR-21 的表达在 NSCLC 中增加，与 SNHG10 的表达呈负相关。在 NSCLC 细胞中，SNHG10 的过表达导致 miR-21 基因甲基化增加和 miR-21 表达降低。此外，SNHG10 的过表达减弱了 miR-21 过表达对细胞增殖的增强作用。
SNHG10 可能通过调节 miR-21 基因甲基化参与 NSCLC 细胞增殖。
