Sakai S, Yoshii A, Sakurai S, Horii K, Nagasuna O
Division of Chemical Engineering, Department of Materials Engineering Science, Graduate School of Engineering Science, Osaka University, 1-3 Machikaneyama-Cho, Toyonaka, Osaka, 560-8531, Japan.
Nagasuna Mayu Inc., Kyotango, Kyoto, 629-3101, Japan.
Mater Today Bio. 2020 Sep 19;8:100078. doi: 10.1016/j.mtbio.2020.100078. eCollection 2020 Sep.
Here, we investigated the usefulness of silk fibroin nanofibers obtained via mechanical grinding of degummed silkworm silk fibers as an additive in bioinks for extrusion three-dimensional (3D) bioprinting of cell-laden constructs. The nanofibers could be sterilized by autoclaving, and addition of the nanofibers improved the shear thinning of polymeric aqueous solutions, independent of electric charge and the content of cross-linkable moieties in the polymers. The addition of nanofibers to bioinks resulted in the fabrication of hydrogel constructs with higher fidelity to blueprints. Mammalian cells in the constructs showed >85% viability independent of the presence of nanofibers. The nanofibers did not affect the morphologies of enclosed cells. These results demonstrate the great potential of silk fibroin nanofibers obtained via mechanical grinding of degummed silkworm silk fibers as an additive in bioinks for extrusion 3D bioprinting.
在此，我们研究了通过对脱胶蚕丝纤维进行机械研磨获得的丝素蛋白纳米纤维作为添加剂用于生物墨水以挤出载有细胞的构建体的三维（3D）生物打印的效用。这些纳米纤维可通过高压灭菌进行消毒，并且添加纳米纤维可改善聚合物水溶液的剪切变稀特性，这与聚合物中的电荷和可交联部分的含量无关。向生物墨水中添加纳米纤维可制造出与蓝图具有更高保真度的水凝胶构建体。构建体中的哺乳动物细胞显示出>85%的活力，与纳米纤维的存在无关。纳米纤维不影响包封细胞的形态。这些结果证明了通过对脱胶蚕丝纤维进行机械研磨获得的丝素蛋白纳米纤维作为添加剂用于生物墨水以进行挤出3D生物打印具有巨大潜力。
