Department of Biochemistry, Molecular Biology, and Biophysics, BioTechnology Institute, University of Minnesota, Twin-Cities, Saint Paul, Minnesota, USA; and Cell Biology, and Development, University of Minnesota, Twin-Cities, Saint Paul, Minnesota, USA.
Department of Genetics, Cell Biology, and Development, University of Minnesota, Twin-Cities, Saint Paul, Minnesota, USA.
CRISPR J. 2020 Oct;3(5):350-364. doi: 10.1089/crispr.2020.0064.
CRISPR-Cas-based transcriptional activators allow genetic engineers to specifically induce expression of one or many target genes . Here we review the many design variations of these versatile tools and compare their effectiveness in different eukaryotic systems. Lastly, we highlight several applications of programmable transcriptional activation to interrogate and engineer complex biological processes.
基于 CRISPR-Cas 的转录激活子使遗传工程师能够特异性地诱导一个或多个靶基因的表达。在这里，我们回顾了这些多功能工具的许多设计变化，并比较了它们在不同真核系统中的有效性。最后，我们强调了可编程转录激活在探究和工程复杂生物过程中的几个应用。
