Department of Orthopedics, Movement System Injury and Repair Research Center, Xiangya Hospital, Central South University, Changsha, Hunan 410008, China.
Angmedicine Research Center of Central South University, Changsha, Hunan 410008, China.
Sci Adv. 2020 Oct 23;6(43). doi: 10.1126/sciadv.aba0942. Print 2020 Oct.
Poor wound healing after diabetes or extensive burn remains a challenging problem. Recently, we presented a physical approach to fabricate ultrasmall silver particles from Ångstrom scale to nanoscale and determined the antitumor efficacy of Ångstrom-scale silver particles (AgÅPs) in the smallest size range. Here we used the medium-sized AgÅPs (65.9 ± 31.6 Å) to prepare carbomer gel incorporated with these larger AgÅPs (L-AgÅPs-gel) and demonstrated the potent broad-spectrum antibacterial activity of L-AgÅPs-gel without obvious toxicity on wound healing-related cells. Induction of reactive oxygen species contributed to L-AgÅPs-gel-induced bacterial death. Topical application of L-AgÅPs-gel to mouse skin triggered much stronger effects than the commercial silver nanoparticles (AgNPs)-gel to prevent bacterial colonization, reduce inflammation, and accelerate diabetic and burn wound healing. L-AgÅPs were distributed locally in skin without inducing systemic toxicities. This study suggests that L-AgÅPs-gel represents an effective and safe antibacterial and anti-inflammatory material for wound therapy.
糖尿病或大面积烧伤后的伤口愈合不良仍然是一个具有挑战性的问题。最近，我们提出了一种物理方法，可将银纳米颗粒从埃尺度制备到纳米尺度，并确定了在最小尺寸范围内埃尺度银颗粒（AgÅPs）的抗肿瘤功效。在这里，我们使用中尺度 AgÅPs（65.9 ± 31.6 Å）来制备载有这些较大 AgÅPs 的卡波姆凝胶（L-AgÅPs-gel），并证明了 L-AgÅPs-gel 具有广谱强效的抗菌活性，而对与伤口愈合相关的细胞没有明显的毒性。活性氧的诱导有助于 L-AgÅPs-gel 诱导的细菌死亡。将 L-AgÅPs-gel 局部应用于小鼠皮肤可引发比商业纳米银颗粒（AgNPs）-凝胶更强的作用，以防止细菌定植、减少炎症并加速糖尿病和烧伤伤口愈合。L-AgÅPs 局部分布于皮肤中，不会引起全身毒性。这项研究表明，L-AgÅPs-gel 是一种用于伤口治疗的有效且安全的抗菌和抗炎材料。
