Deng Yinhe, Li Quanjiang, Li Menglin, Han Tiantian, Li Guixian, Liu Qiong
College of First Clinical Medical, Guangzhou University of Chinese Medicine, Guangzhou 51000, China.
International Medical Department, The First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou 51000, China.
Evid Based Complement Alternat Med. 2020 Oct 12;2020:2421916. doi: 10.1155/2020/2421916. eCollection 2020.
Sang-Xing-Zhi-Ke-Fang (SXZKF) demonstrates good therapeutic effect against pharyngitis. Nevertheless, the pharmacological mechanism underlying its effectiveness is still unclear.
To investigate the underlying mechanisms of SXZKF against pharyngitis using network pharmacology method.
Bioactive ingredients of SXZKF were collected and screened using published literature and two public databases. Using four public databases, the overlapping genes between these bioactive compound-related and pharyngitis-related genes were identified by Venn diagram. Protein-protein interaction (PPI) was obtained using "Search Tool for the Retrieval of Interacting Genes (STRING)" database. "Database for Annotation, Visualization, and Integrated Discovery ver. 6.8 (DAVID 6.8)" was used to perform Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis to explore the molecular mechanisms of SXZKF against pharyngitis. Finally, Cytoscape 3.7.2 software was used to construct and visualize the networks.
A total of 102 bioactive compounds were identified. Among them, 886 compounds-related and 6258 pharyngitis-related genes were identified, including 387 overlapping genes. Sixty-three core targets were obtained, including ALB, PPAR, MAPK3, EGF, and PTGS2. Signaling pathways closely related to mechanisms of SXZKF for pharyngitis were identified, including serotonergic synapse, VEGF signaling pathway, Fc epsilon RI signaling pathway, Ras signaling pathway, MAPK signaling pathway, and influenza A.
This is the first identification of in-depth study of SXZKF against pharyngitis using network pharmacology. This new evidence could be informative in providing new support on the clinical effects of SXZKF on pharyngitis and for the development of personalized medicine for pharyngitis.
桑杏止咳方（SXZKF）对咽炎具有良好的治疗效果。然而，其疗效背后的药理机制仍不清楚。
采用网络药理学方法探讨SXZKF治疗咽炎的潜在机制。
利用已发表的文献和两个公共数据库收集并筛选SXZKF的生物活性成分。使用四个公共数据库，通过维恩图确定这些生物活性化合物相关基因与咽炎相关基因之间的重叠基因。利用“检索相互作用基因的搜索工具（STRING）”数据库获得蛋白质-蛋白质相互作用（PPI）。使用“注释、可视化和综合发现数据库6.8版（DAVID 6.8）”进行京都基因与基因组百科全书（KEGG）通路富集分析，以探索SXZKF治疗咽炎的分子机制。最后，使用Cytoscape 3.7.2软件构建并可视化网络。
共鉴定出102种生物活性化合物。其中，鉴定出886个化合物相关基因和6258个咽炎相关基因，包括387个重叠基因。获得63个核心靶点，包括ALB、PPAR、MAPK3、EGF和PTGS2。确定了与SXZKF治疗咽炎机制密切相关的信号通路，包括5-羟色胺能突触、VEGF信号通路、FcεRI信号通路、Ras信号通路、MAPK信号通路和甲型流感。
这是首次使用网络药理学对SXZKF治疗咽炎进行深入研究的鉴定。这一新证据可为SXZKF对咽炎的临床疗效提供新的支持，并为咽炎个性化药物的开发提供参考。
