Department of Genetics, Yale Stem Cell Center, Yale School of Medicine, New Haven, CT 06520, USA.
Technical Contact.
STAR Protoc. 2020 Jun 19;1(1). doi: 10.1016/j.xpro.2019.100001. Epub 2020 Jun 4.
Thalamus is a critical information relay hub in the cortex; its malfunction causes multiple neurological and psychiatric disorders. However, there are no model systems to study the development and function of human thalamus. Here, we present a protocol to generate regionally specified human brain organoids that recapitulate the development of the thalamus using human pluripotent stem cells (hPSCs). Thalamic organoids can be used to study human thalamus development, to model related diseases, and to discover potential therapeutics. For complete information on human thalamic organoids and their application, please refer to the paper by Xiang et al. (2019).
丘脑是大脑皮层中关键的信息中继枢纽；其功能障碍会导致多种神经和精神疾病。但是，目前还没有模型系统可以研究人类丘脑的发育和功能。在这里，我们提供了一个使用人类多能干细胞（hPSC）生成具有区域特异性的人类大脑类器官的方案，该方案可重现丘脑的发育过程。丘脑类器官可用于研究人类丘脑的发育、模拟相关疾病，并发现潜在的治疗方法。有关人类丘脑类器官及其应用的完整信息，请参考 Xiang 等人的论文（2019 年）。
