Husby G, Williams R C, Watt R A, West S G, Tung K S
Department of Medicine, University of New Mexico School of Medicine, Albuquerque 87131.
Clin Exp Immunol. 1987 Sep;69(3):493-500.
Frozen sections of kidney tissue from 12 patients with systemic lupus erythematosus were examined for the expression of oncogene proteins. Polyclonal rabbit antibody to c-myc peptide or to whole 65,000 D c-myc protein, was used to identify c-myc protein within tissue sections. Patterns of nuclear staining in the Hep-2 cell line were similar to those seen with monospecific human SLE sera showing anti-Sm reactivity. In addition c-myc staining was not abolished by prior incubation of tissue sections with human serum containing anti-Sm antibodies. Five of 12 kidney biopsy tissues from patients with systemic lupus erythematosus (SLE) showed positive speckled c-myc protein staining within nuclei of monocyte/macrophage cells of glomerular tufts. Positive staining was in all instances completely abolished by prior absorption of anti-c-myc antibody with c-myc protein. No c-myc protein was identified within SLE immune complex deposits.
对12例系统性红斑狼疮患者的肾组织冰冻切片进行癌基因蛋白表达检测。使用抗c-myc肽或完整的65000D c-myc蛋白的多克隆兔抗体来识别组织切片中的c-myc蛋白。Hep-2细胞系中的核染色模式与显示抗Sm反应性的单特异性人SLE血清所见相似。此外，用含抗Sm抗体的人血清预先孵育组织切片后，c-myc染色并未消除。12例系统性红斑狼疮（SLE）患者的肾活检组织中有5例在肾小球丛单核细胞/巨噬细胞的细胞核内显示出阳性斑点状c-myc蛋白染色。在所有情况下，用c-myc蛋白预先吸收抗c-myc抗体后，阳性染色完全消除。在SLE免疫复合物沉积物中未鉴定出c-myc蛋白。
