SARS-CoV-2 的进化分析：非结构蛋白 6（NSP6）的突变如何影响病毒自噬。
Evolutionary analysis of SARS-CoV-2: how mutation of Non-Structural Protein 6 (NSP6) could affect viral autophagy.
机构信息
Unit of Medical Statistics and Molecular Epidemiology, University Campus Bio-Medico of Rome, Via Álvaro del Portillo, 21, Rome 00128, Italy.
Unit of Clinical Laboratory Science, University Campus Bio-Medico of Rome, Via Álvaro del Portillo, 21, Rome 00128, Italy.
出版信息
J Infect. 2020 Jul;81(1):e24-e27. doi: 10.1016/j.jinf.2020.03.058. Epub 2020 Apr 10.
BACKGROUND
SARS-CoV-2 is a new coronavirus that has spread globally, infecting more than 150000 people, and being declared pandemic by the WHO. We provide here bio-informatic, evolutionary analysis of 351 available sequences of its genome with the aim of mapping genome structural variations and the patterns of selection.
METHODS
A Maximum likelihood tree has been built and selective pressure has been investigated in order to find any mutation developed during the SARS-CoV-2 epidemic that could potentially affect clinical evolution of the infection.
FINDING
We have found in more recent isolates the presence of two mutations affecting the Non-Structural Protein 6 (NSP6) and the Open Reding Frame10 (ORF 10) adjacent regions. Amino acidic change stability analysis suggests both mutations could confer lower stability of the protein structures.
INTERPRETATION
One of the two mutations, likely developed within the genome during virus spread, could affect virus intracellular survival. Genome follow-up of SARS-CoV-2 spread is urgently needed in order to identify mutations that could significantly modify virus pathogenicity.
背景
SARS-CoV-2 是一种新型冠状病毒，已在全球范围内传播，感染了超过 15 万人，并被世界卫生组织宣布为大流行。我们在此提供其基因组的 351 个可用序列的生物信息学和进化分析，旨在绘制基因组结构变异和选择模式。
方法
构建了最大似然树，并研究了选择压力，以寻找 SARS-CoV-2 流行期间可能影响感染临床演变的任何突变。
发现
我们在最近的分离株中发现了两个影响非结构蛋白 6（NSP6）和开放阅读框 10（ORF10）相邻区域的突变。氨基酸变化稳定性分析表明，这两个突变都可能导致蛋白质结构稳定性降低。
解释
这两个突变中的一个可能是在病毒传播过程中在基因组内发展起来的，可能会影响病毒的细胞内生存。迫切需要对 SARS-CoV-2 传播的基因组进行跟踪，以识别可能显著改变病毒致病性的突变。
Zotero 插件