Zeng Jianrong, Liu Jianzhong, Lu Changhua, Ou Xiaohua, Luo Keke, Li Chengmei, He Mengling, Zhang Hongyi, Yan Hanjing
College of Traditional Chinese Medicine, Guangdong Pharmaceutical University, Guangzhou, China.
Comprehensive Experimental Station of Guangzhou, Chines: Material Medica, China Agriculture Research System (Cars-21-16), Guangzhou, China.
Front Microbiol. 2020 Oct 8;11:579719. doi: 10.3389/fmicb.2020.579719. eCollection 2020.
Continuous cropping (CC) restricts the development of the medicinal plant cultivation industry because it alters soil properties and the soil microbial micro-ecological environment. It can also lead to reductions in the chemical contents of medicinal plants. In this study, we intercropped continuously cropped (patchouli) with turmeric or ginger. High-throughput sequencing was used to study the soil bacteria and fungi. Community composition, diversity, colony structure, and colony differences were also analyzed. A redundancy analysis (RDA) was used to study the interactions between soil physical and chemical factors, and the bacteria and fungi. The correlations between the soil community and the soil physical and chemical properties were also investigated. The results showed that intercropping turmeric and ginger with patchouli can improve soil microbial abundance, diversity, and community structure by boosting the number of dominant bacteria, and by improving soil bacterial metabolism and the activities of soil enzymes. They also modify the soil physical and chemical properties through changes in enzyme activity, soil pH, and soil exchangeable Ca (Ca). In summary, turmeric and ginger affect the distribution of dominant bacteria, and increase the contents of the active ingredient in patchouli. The results from this study suggested that the problems associated with continuously cropping patchouli can be ameliorated by intercropping it with turmeric and ginger.
连作限制了药用植物种植业的发展,因为它会改变土壤性质和土壤微生物微生态环境。连作还会导致药用植物化学含量降低。在本研究中,我们将连作的广藿香与姜黄或生姜进行间作。采用高通量测序技术研究土壤细菌和真菌。同时分析了群落组成、多样性、菌落结构和菌落差异。利用冗余分析(RDA)研究土壤理化因子与细菌和真菌之间的相互作用。还研究了土壤群落与土壤理化性质之间的相关性。结果表明,将姜黄和生姜与广藿香间作,可以通过增加优势细菌数量、改善土壤细菌代谢和土壤酶活性来提高土壤微生物丰度、多样性和群落结构。它们还通过酶活性、土壤pH值和土壤交换性钙(Ca)的变化来改变土壤理化性质。总之,姜黄和生姜影响优势细菌的分布,并增加广藿香中活性成分的含量。本研究结果表明,将广藿香与姜黄和生姜间作可以改善连作带来的问题。