Pauletto Marianna, Giantin Mery, Tolosi Roberta, Bassan Irene, Barbarossa Andrea, Zaghini Anna, Dacasto Mauro
Division of Pharmacology and Toxicology, Department of Comparative Biomedicine and Food Science, University of Padova, viale dell'Università 16, Legnaro, 35020 Padova, Italy.
Department of Veterinary Medical Sciences, University of Bologna, Via Tolara di Sopra 50, Ozzano dell'Emilia, 40064 Bologna, Italy.
Antioxidants (Basel). 2020 Oct 29;9(11):1059. doi: 10.3390/antiox9111059.
Aflatoxin B1 (AFB1) toxicity in livestock and human beings is a major economic and health concern. Natural polyphenolic substances with antioxidant properties have proven to be effective in ameliorating AFB1-induced toxicity. Here we assessed the potential anti-AFB1 activity of curcumin (pure curcumin, C, and curcumin from , CL) in a bovine fetal hepatocyte-derived cell line (BFH12). First, we measured viability of cells exposed to AFB1 in presence or absence of curcumin treatment. Then, we explored all the transcriptional changes occurring in AFB1-exposed cells cotreated with curcumin. Results demonstrated that curcumin is effective in reducing AFB1-induced toxicity, decreasing cells mortality by approximately 30%. C and CL induced similar transcriptional changes in BFH12 exposed to AFB1, yet C treatment resulted in a larger number of significant genes compared to CL. The mitigating effects of curcuminoids towards AFB1 toxicity were mainly related to molecular pathways associated with antioxidant and anti-inflammatory response, cancer, and drug metabolism. Investigating mRNA changes induced by curcumin in cattle BFH12 cells exposed to AFB1 will help us to better characterize possible tools to reduce its consequences in this susceptible and economically important food-producing species.
黄曲霉毒素B1（AFB1）对家畜和人类的毒性是一个重大的经济和健康问题。具有抗氧化特性的天然多酚类物质已被证明能有效改善AFB1诱导的毒性。在此，我们评估了姜黄素（纯姜黄素，C，以及来自[具体来源未提及]的姜黄素，CL）在牛胎儿肝细胞系（BFH12）中的潜在抗AFB1活性。首先，我们测量了在有或没有姜黄素处理的情况下，暴露于AFB1的细胞的活力。然后，我们探究了与姜黄素共同处理的AFB1暴露细胞中发生的所有转录变化。结果表明，姜黄素能有效降低AFB1诱导的毒性，使细胞死亡率降低约30%。C和CL在暴露于AFB1的BFH12中诱导了相似的转录变化，但与CL相比，C处理导致了更多数量的显著基因。姜黄素类化合物对AFB1毒性的减轻作用主要与抗氧化和抗炎反应、癌症以及药物代谢相关的分子途径有关。研究姜黄素在暴露于AFB1的牛BFH12细胞中诱导的mRNA变化，将有助于我们更好地确定可能的工具，以减少其对这种易感且具有重要经济意义的产粮物种的影响。
