Center for Microbial Ecology and Technology (CMET), Department of Biochemical and Microbial Technology, Ghent University, Coupure Links 653, Ghent, 9000, Belgium.
INVE Technologies NV, Hoogveld 93, Dendermonde, 9200, Belgium.
Environ Microbiol. 2021 Jan;23(1):281-298. doi: 10.1111/1462-2920.15310. Epub 2020 Nov 18.
The development of effective management strategies to reduce the occurrence of diseases in aquaculture is hampered by the limited knowledge on the microbial ecology of these systems. In this study, the dynamics and dominant community assembly processes in the rearing water of Litopenaeus vannamei larviculture tanks were determined. Additionally, the contribution of peripheral microbiomes, such as those of live and dry feeds, to the rearing water microbiome were quantified. The community assembly in the hatchery rearing water over time was dominated by stochasticity, which explains the observed heterogeneity between replicate cultivations. The community undergoes two shifts that match with the dynamics of the algal abundances in the rearing water. Source tracking analysis revealed that 37% of all bacteria in the hatchery rearing water were introduced either by the live or dry feeds, or during water exchanges. The contribution of the microbiome from the algae was the largest, followed by that of the Artemia, the exchange water and the dry feeds. Our findings provide fundamental knowledge on the assembly processes and dynamics of rearing water microbiomes and illustrate the crucial role of these peripheral microbiomes in maintaining health-promoting rearing water microbiomes.
养殖水体中的疾病发生难以得到有效控制，这是由于我们对这些系统的微生物生态学了解有限。本研究旨在确定凡纳滨对虾育苗水体的动态变化和主要群落组装过程，并定量分析了周边微生物组（如活饲料和干饲料）对育苗水微生物组的贡献。研究发现，随着时间的推移，孵化场养殖水的群落组装主要受随机性的影响，这解释了养殖过程中观察到的异质性。群落经历了两次转变，与养殖水中藻类丰度的动态变化相吻合。来源追踪分析表明，孵化场养殖水中的 37%的细菌是由活饲料或干饲料或换水过程引入的。藻类的微生物组贡献最大，其次是卤虫、交换水和干饲料。本研究结果为养殖水微生物组的组装过程和动态变化提供了基础认识，并说明了这些周边微生物组在维持健康促进养殖水微生物组方面的关键作用。
