Department of Chemistry, Integrative Biosciences (Ibio), Wayne State University, Karmanos Cancer Institute (KCI), Detroit, Michigan 48202, United States.
Department of Radiology, Vanderbilt University Institute of Imaging Science (VUIIS), Nashville, Tennessee 37232, United States.
Anal Chem. 2020 Dec 1;92(23):15280-15284. doi: 10.1021/acs.analchem.0c03358. Epub 2020 Nov 10.
We present an automated parahydrogen generator (ParaSun) for clinical-scale applications in parahydrogen-induced polarization (PHIP) and signal amplification by reversible exchange (SABRE) at high pressures. The device employs a vacuum-pumped, Sunpower cryo-cooler (typically employed for cooling cellular network antennas) to achieve up to ∼87% parahydrogen enrichment at a temperature as low as ∼40 K and a maximum outlet pressure of ∼490 PSI. The device reaches the target temperature set-point in under 1 h. It employs a FeO(OH) catalyst for the ortho- to para-state conversion. A mass-flow controller (MFC) facilitates the controlled flow of H gas at a rate of 150 standard cubic centimeters per minute (sccm). This design bridges the gap between rudimentary 50% enrichment liquid-N baths and far costlier, near-unity-enrichment configurations employing high-H throughputs and <25 K temperatures. The design presented here should be of interest for those pursuing a wide variety of PHIP applications, including those involving the production of inhalable or injectable hyperpolarized contrast agents for biomedical imaging.
我们提出了一种自动化的重氢发生器（ParaSun），用于在临床规模的重氢诱导极化（PHIP）和通过可逆交换进行信号放大（SABRE）中应用于高压环境。该设备采用了一种经过真空抽气的 Sunpower 制冷机（通常用于冷却蜂窝网络天线），可在低至约 40 K 的温度下实现高达约 87%的重氢富集，最大出口压力可达约 490 PSI。该设备在不到 1 小时内即可达到目标温度设定点。它采用 FeO(OH)催化剂实现正氢到仲氢的转化。质量流量控制器（MFC）可实现以 150 标准立方厘米每分钟（sccm）的速率控制 H 气体的流量。该设计填补了基础的 50%富集液氦浴与成本更高、接近 100%富集、采用高 H 流量和 <25 K 温度的设备之间的差距。对于那些从事广泛的 PHIP 应用的人来说，包括生产可吸入或可注射的用于生物医学成像的超极化对比剂的应用，这里提出的设计应该是有吸引力的。
