Vladić Jelena, Ćebović Tatjana, Vidović Senka, Jokić Stela
Department of Biotechnology and Pharmaceutical Engineering, Faculty of Technology, University of Novi Sad, Bulevar Cara Lazara 1, 21000 Novi Sad, Serbia.
Department of Biochemistry, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000 Novi Sad, Serbia.
Plants (Basel). 2020 Nov 10;9(11):1532. doi: 10.3390/plants9111532.
herbal species belongs to aromatic medicinal plants with a significant place in traditional medicine. However, products produced with conventional procedures do not meet the requirements of the modern market which include environmentally-safe processes that provide quality, safe, and standardized products. In this study, the antiproliferative activity of extracts obtained by supercritical carbon dioxide and solid-liquid extraction followed by spray drying was investigated using the in vivo model of Ehrlich ascites carcinoma (EAC) in mice. The impact of two concentrations of extracts on the growth of tumor and the redox status of malignant cells was monitored. It was determined that the extracts induced oxidative stress in the malignant cells which was confirmed by the changes in activity of biochemical indicators of oxidative stress. The posttreatment was not an efficient approach, while the extracts applied as pretreatment and treatment resulted in an increase in the xanthine oxidase (XOD) activity, a decrease in catalase (CAT) activity, and an increase in the intensity of lipid peroxidation (LPx). Furthermore, a decrease in the values of reduced glutathione (GSH) and an increase in glutathione reductase (GR) and glutathione peroxidase (GSHPx) in EAC cells were recorded.
该草本植物属于芳香药用植物，在传统医学中占有重要地位。然而，采用传统工艺生产的产品无法满足现代市场的要求，现代市场需要环保型工艺来提供优质、安全和标准化的产品。在本研究中，使用小鼠艾氏腹水癌（EAC）体内模型，研究了通过超临界二氧化碳和固液萃取后喷雾干燥获得的提取物的抗增殖活性。监测了两种浓度提取物对肿瘤生长和恶性细胞氧化还原状态的影响。结果表明，提取物在恶性细胞中诱导了氧化应激，这通过氧化应激生化指标活性的变化得到证实。后处理不是一种有效的方法，而作为预处理和处理应用的提取物导致黄嘌呤氧化酶（XOD）活性增加、过氧化氢酶（CAT）活性降低以及脂质过氧化（LPx）强度增加。此外，还记录到EAC细胞中还原型谷胱甘肽（GSH）值降低，谷胱甘肽还原酶（GR）和谷胱甘肽过氧化物酶（GSHPx）增加。
