Vladić Jelena, Duarte Ana Rita C, Radman Sanja, Simić Siniša, Jerković Igor
Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.
Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000 Novi Sad, Serbia.
Plants (Basel). 2021 Dec 25;11(1):54. doi: 10.3390/plants11010054.
The goal of the study was to establish a procedure for improving the efficiency of supercritical carbon dioxide (scCO) extraction of L. spp. (Greek oregano) and enhancing the quality of obtained extracts. Microwave and enzymatic pretreatments of the plant material were applied prior to the scCO extraction. It was determined that the microwave pretreatment with irradiation power 360 W during 2 min accelerated the extraction of lipophilic compounds and provided a twofold higher extraction yield compared to the control. Moreover, this pretreatment also led to an increase in oxygenated monoterpenes content and the most dominant component carvacrol, as well as the extracts' antioxidant activity. The enzymatic pretreatment caused a significant increase in the extraction yield and the attainment of the extract with the most potent antioxidant properties. Coupling the pretreatments with scCO extraction improves the process of obtaining high value lipophilic products of oregano in terms of utilization of the plant material, acceleration of the extraction with the possibility to adjust its selectivity and quality of extracts, and enhancement of biological activity.
该研究的目标是建立一种程序，以提高超临界二氧化碳（scCO）萃取希腊牛至（L. spp.）的效率，并提升所得提取物的质量。在scCO萃取之前，对植物材料进行了微波和酶预处理。结果表明，在360 W辐射功率下进行2分钟的微波预处理加速了亲脂性化合物的萃取，与对照相比，萃取产率提高了两倍。此外，这种预处理还导致了氧化单萜含量和最主要成分香芹酚的增加，以及提取物抗氧化活性的提高。酶预处理使萃取产率显著提高，并获得了具有最强抗氧化性能的提取物。将预处理与scCO萃取相结合，在植物材料的利用、加速萃取并可调节其选择性和提取物质量以及增强生物活性方面，改善了获得高价值牛至亲脂性产品的过程。
