Zeng Yuyang, Liang Demei, Zheng Pimiao, Zhang Yanfang, Wang Zile, Mari Ghulam Mujtaba, Jiang Haiyang
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
J Dairy Sci. 2021 Jan;104(1):126-133. doi: 10.3168/jds.2020-18987. Epub 2020 Nov 12.
In this study, we developed a novel, simple, rapid, and low-cost colloidal gold-based immunochromatography method, with filter paper replacing nitrocellulose membrane as the substrate. To obtain adequately immobilized protein, chitosan was used to functionalize the filter paper. After conditions and parameters were optimized, the novel immunochromatography method was applied for detection of sulfonamide residues in milk. Quantitative detection was accomplished using a smartphone and Photoshop software (Adobe Inc., San Jose, CA), allowing us to screen 13 sulfonamides with a limit of detection ranging from 0.42 to 8.64 μg/L and recovery ranging from 88.2 to 116.9% in milk. The proposed novel method performed similarly to the conventional method that uses a nitrocellulose membrane as the transport medium, and it had lower cost and better usability because of the inexpensive and easily available filter paper.
在本研究中，我们开发了一种新颖、简单、快速且低成本的基于胶体金的免疫层析方法，用滤纸替代硝酸纤维素膜作为基质。为了获得充分固定的蛋白质，使用壳聚糖对滤纸进行功能化处理。在优化条件和参数后，将这种新型免疫层析方法应用于牛奶中磺胺类药物残留的检测。使用智能手机和Photoshop软件（Adobe公司，加利福尼亚州圣何塞）完成定量检测，使我们能够筛选出13种磺胺类药物，其在牛奶中的检测限为0.42至8.64μg/L，回收率为88.2%至116.9%。所提出的新方法与使用硝酸纤维素膜作为传输介质的传统方法表现相似，并且由于滤纸价格低廉且易于获得，该方法成本更低且可用性更好。
