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用于检测弓形虫抗体的快速筛查免疫测定法的评估

Evaluation of a rapid screening immunoassay for antibodies to Toxoplasma gondii.

作者信息

Smith S B, Repetti C F

机构信息

Department of Immunopathology, American Medical Laboratories, Inc., Fairfax, Virginia 22030.

出版信息

J Clin Microbiol. 1987 Nov;25(11):2207-8. doi: 10.1128/jcm.25.11.2207-2208.1987.

DOI:10.1128/jcm.25.11.2207-2208.1987

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC269442/

Abstract

A total of 349 human serum samples were examined for anti-Toxoplasma antibodies by the Murex Single Use Diagnostic System (SUDS) qualitative screening test, indirect hemagglutination assay (IHA), and indirect immunofluorescence assay (IFA). Concordant results with SUDS and IHA were obtained for 91.9% of serum samples; 8.9% were SUDS+ and IHA-; none were SUDS- and IHA+. Comparison of the SUDS assay with IFA showed a concordance of 95.3%, with a sensitivity of 97.5% and a specificity of 94.7%. Moreover, the positive and negative predictive values were 84.9 and 99.2%, respectively, when results of the SUDS assay and IFA were compared. The SUDS assay is a rapid, simple test requiring no instrumentation and can be performed on 50 microliters of serum, features which make this an excellent screening test for detecting anti-Toxoplasma antibodies, particularly in outpatient settings.

摘要

采用Murex一次性诊断系统（SUDS）定性筛查试验、间接血凝试验（IHA）和间接免疫荧光试验（IFA）对349份人血清样本进行抗弓形虫抗体检测。91.9%的血清样本SUDS和IHA结果一致；8.9%的样本SUDS阳性而IHA阴性；无SUDS阴性而IHA阳性的样本。SUDS试验与IFA比较，一致性为95.3%，敏感性为97.5%，特异性为94.7%。此外，比较SUDS试验和IFA结果时，阳性预测值和阴性预测值分别为84.9%和99.2%。SUDS试验是一种快速、简单的检测方法，无需仪器设备，50微升血清即可检测，这些特点使其成为检测抗弓形虫抗体的优秀筛查试验，尤其适用于门诊环境。