Shahriari-Khalaji Mina, Hong Siyi, Hu Gaoquan, Ji Ying, Hong Feng F
Microbiological Engineering and Industrial Biotechnology Group, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai 201620, China.
Scientific Research Base of Bacterial Nanofiber Manufacturing and Composite Technology, China Textile Engineering Society, Shanghai 201620, China.
Polymers (Basel). 2020 Nov 13;12(11):2683. doi: 10.3390/polym12112683.
Alginate (Alg) and bacterial nanocellulose (BNC) have exhibited great potential in biomedical applications, especially wound dressing. Non-toxicity and a moisture-maintaining nature are common features making them favorable for functional dressing fabrication. BNC is a natural biopolymer that promotes major advances to the current and future biomedical materials, especially in a flat or tubular membrane form with excellent mechanical strength at hydrated state. The main drawback limiting wide applications of both BNC and Alg is the lack of antibacterial activity, furthermore, the inherent poor mechanical property of Alg leads to the requirement of a secondary dressing in clinical treatment. To fabricate composite dressings with antibacterial activity and better mechanical properties, sodium alginate was efficiently incorporated into the BNC matrix using a time-saving vacuum suction method followed by cross-linking through immersion in separate solutions of six cations (manganese, cobalt, copper, zinc, silver, and cerium). The results showed the fabricated composites had not only pH-responsive antibacterial activities but also improved mechanical properties, which are capable of acting as smart dressings. All composites showed non-toxicity toward fibroblast cells. Rat model evaluation showed the skin wounds covered by the dressings healed faster than by BNC.
海藻酸盐（Alg）和细菌纳米纤维素（BNC）在生物医学应用中展现出了巨大潜力，尤其是在伤口敷料方面。无毒和保湿特性是它们有利于制造功能性敷料的共同特点。BNC是一种天然生物聚合物，为当前和未来的生物医学材料带来了重大进展，特别是呈扁平或管状膜形式时，在水合状态下具有优异的机械强度。限制BNC和Alg广泛应用的主要缺点是缺乏抗菌活性，此外，Alg固有的机械性能较差导致在临床治疗中需要二次敷料。为了制备具有抗菌活性和更好机械性能的复合敷料，采用省时的真空抽吸法将海藻酸钠有效地掺入BNC基质中，然后通过浸入六种阳离子（锰、钴、铜、锌、银和铈）的单独溶液中进行交联。结果表明，制备的复合材料不仅具有pH响应抗菌活性，而且机械性能得到改善，能够作为智能敷料。所有复合材料对成纤维细胞均无毒性。大鼠模型评估表明，用这些敷料覆盖的皮肤伤口比用BNC覆盖的愈合更快。
