School of Molecular and Life Sciences, Curtin University, Bentley, WA, Australia.
Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet, Bangladesh.
J Fish Dis. 2021 May;44(5):591-599. doi: 10.1111/jfd.13304. Epub 2020 Nov 18.
Vibriosis caused by luminous Vibrio species is one of the biggest challenges to shrimp industry in Bangladesh. This study aimed to characterize whole microbial communities from Vibrio-infected black tiger shrimp (Penaeus monodon) using 16S rRNA-based amplicon sequencing. A total of 36 disease-free and infected shrimp were collected from six different hatcheries in Bagerhat, Bangladesh. A final pool of 12 samples (n = 6) was created by homogenization of the hepatopancreas samples from three shrimps collected from each hatchery for the same group. The amplicon sequencing data revealed significant (p < .05) decrease of alpha diversity measurements and subsequent effects (p < .05) on the hepatopancreas microbiota in the infected group, compared to control shrimp. Proteobateria and Aeromonas were the most dominant bacteria at phylum and genus level in both groups and identified as core microbiota in the community. Two bacterial groups at phyla level and eight at genus level were found associated with the alteration of hepatopancreas microbial communities and associated gene functions in vibriosis-infected shrimp, revealed by differential abundance and KEGG pathway analysis. The overwhelming abundance of Citroibacter, Shewanella and Candidatus lineages in vibriosis-infected shrimp needs further investigations.
发光弧菌引起的弧菌病是孟加拉国对虾产业面临的最大挑战之一。本研究旨在通过基于 16S rRNA 的扩增子测序来描述受弧菌感染的黑虎虾（Penaeus monodon）的整个微生物群落。总共从孟加拉国巴盖尔哈特的六个不同孵化场收集了 36 只无病和感染的虾。通过对每个孵化场收集的三只虾的肝胰腺样本进行均质化，共创建了 12 个最终样本（n=6），用于同一组。与对照组虾相比，扩增子测序数据显示，感染组的 alpha 多样性测量值显著（p<.05）下降，随后对肝胰腺微生物群产生影响（p<.05）。在两组中，厚壁菌门和气单胞菌属是最主要的细菌门和属，并且被鉴定为群落中的核心微生物群。在感染弧菌的虾中，通过差异丰度和 KEGG 途径分析，发现有两个细菌门和八个细菌属与肝胰腺微生物群落的改变及其相关基因功能有关。在感染弧菌的虾中，Citroibacter、Shewanella 和 Candidatus 菌属的丰度过高，需要进一步研究。
