Department of Microbiology and Immunology, Tulane University, 1430 Tulane Avenue, JBJ568, New Orleans, LA, 70112, USA.
Ansun Biopharma, San Diego, CA, 92121, USA.
Virol J. 2020 Nov 26;17(1):188. doi: 10.1186/s12985-020-01459-y.
Lassa fever and Ebola are characterized by non-specific initial presentations that can progress to severe multisystem illnesses with high fatality rates. Samples from additional subjects are examined to extend and corroborate biomarkers with prognostic value for these diseases.
Liquid Chromatography Mass Spectrometry metabolomics was used to identify and confirm metabolites disrupted in the blood of Lassa fever and Ebola patients. Authenticated standards are used to confirm the identify of key metabolites.
We confirm prior results by other investigators that the amino acid L-threonine is elevated during Ebola virus infection. L-Threonine is also elevated during Lassa virus infection. We also confirmed that platelet-activating factor (PAF) and molecules with PAF moiety are reduced in the blood of patients with fatal Lassa fever. Similar changes in PAF and PAF-like molecules were not observed in the blood of Ebola patients.
Metabolomics may provide tools to identify pathways that are differentially affected during viral hemorrhagic fevers and guide development of diagnostics to monitor and predict outcome.
拉沙热和埃博拉病毒的初始表现都不具有特异性，可进展为严重的多系统疾病，死亡率很高。对其他受试者的样本进行检查，以扩展和证实具有这些疾病预后价值的生物标志物。
采用液相色谱-质谱代谢组学方法鉴定和确认拉沙热和埃博拉患者血液中失调的代谢物。使用经过验证的标准来确认关键代谢物的身份。
我们确认了其他研究人员的先前结果，即在埃博拉病毒感染期间，氨基酸 L-苏氨酸升高。在拉沙病毒感染期间，L-苏氨酸也升高。我们还证实，在致命性拉沙热患者的血液中，血小板激活因子（PAF）和具有 PAF 部分的分子减少。在埃博拉患者的血液中没有观察到 PAF 和 PAF 样分子的类似变化。
代谢组学可能为鉴定病毒出血热期间受影响的途径提供工具，并为监测和预测结果的诊断工具的开发提供指导。
