School of Industrial Technology, Universiti Sains Malaysia, 11800 Penang, Malaysia.
Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Benef Microbes. 2020 Dec 2;11(8):753-766. doi: 10.3920/BM2019.0200. Epub 2020 Nov 27.
Aging processes affect the brain in many ways, ranging from cellular to functional levels which lead to cognitive decline and increased oxidative stress. The aim of this study was to investigate the potentials of DR7 on brain health including cognitive and memory functions during aging and the impacts of high fat diet during a 12-week period. Male Sprague-Dawley rats were separated into six groups: (1) young animals on normal diet (ND, (2) young animals on a high fat diet (HFD), (3) aged animals on ND, (4) aged animals on HFD, (5) aged animals on HFD and DR7 (10 cfu/day) and (6) aged animals receiving HFD and lovastatin. To induce ageing, all rats in group 3 to 6 were injected sub-cutaneously at 600 mg/kg/day of D-galactose daily. The administration of DR7 has reduced anxiety accompanied by enhanced memory during behavioural assessments in aged-HFD rats (<0.05). Hippocampal concentration of all three pro-inflammatory cytokines were increased during aging but reduced upon administration of both statin and DR7. Expressions of hippocampal neurotransmitters and apoptosis genes showed reduced expressions of indoleamine dioxygenase and P53 accompanied by increased expression of TPH1 in aged- HFD rats administered with DR7, indicating potential effects of DR7 along the pathways of serotonin and oxidative senescence. This study provided an insight into potentials of DR7 as a prospective dietary strategy to improve cognitive functions during aging. This study provided an insight into potentials of DR7 as a prospective dietary strategy to improve cognitive functions during aging.
衰老过程会在多个层面影响大脑，从细胞到功能层面，导致认知能力下降和氧化应激增加。本研究旨在探讨 DR7 在大脑健康方面的潜力，包括衰老过程中的认知和记忆功能，以及在 12 周高脂肪饮食期间的影响。雄性 Sprague-Dawley 大鼠被分为六组：（1）正常饮食的年轻动物（ND），（2）高脂肪饮食的年轻动物（HFD），（3）ND 的老年动物，（4）HFD 的老年动物，（5）HFD 的老年动物和 10 cfu/天的 DR7，（6）接受 HFD 和洛伐他汀的老年动物。为了诱导衰老，第 3 至 6 组的所有大鼠均每天经皮注射 600mg/kg/d 的 D-半乳糖。DR7 的给药减少了老龄-HFD 大鼠在行为评估中伴随的焦虑并增强了记忆（<0.05）。在衰老过程中，所有三种促炎细胞因子在海马中的浓度均增加，但在他汀类药物和 DR7 给药后均降低。海马神经递质和凋亡基因的表达显示，老龄-HFD 大鼠中吲哚胺双加氧酶和 P53 的表达减少，同时 DR7 给药的大鼠中 TPH1 的表达增加，这表明 DR7 可能通过 5-羟色胺和氧化衰老途径发挥作用。本研究深入了解了 DR7 作为一种有前途的饮食策略，可在衰老过程中改善认知功能的潜力。
