Department of Cardiac Surgery, Tianjin Chest Hospital, Tianjin, P. R. China.
Department of Dermatology, Tianjin First Central Hospital, Tianjin, P. R. China.
J Int Med Res. 2020 Nov;48(11):300060520969331. doi: 10.1177/0300060520969331.
Vein graft restenosis (VGR), which appears to be caused by dyslipidemia following vascular transplantation, seriously affects the prognosis and long-term quality of life of patients.
This study analyzed the genetic data of restenosis (VGR group) and non-stenosis (control group) vessels from patients with coronary heart disease post-vascular transplantation and identified hub genes that might be responsible for its occurrence. GSE110398 was downloaded from the Gene Expression Omnibus database. A repeatability test for the GSE110398 dataset was performed using R language. This included the identification of differentially expressed genes (DEGs), enrichment analysis via Metascape software, pathway enrichment analysis, and construction of a protein-protein interaction network and a hub gene network.
Twenty-four DEGs were identified between VGR and control groups. The four most important hub genes (, , , and ) were identified, and Pearson's correlation coefficient showed that and were significantly correlated with VGR. could also sensitively predict VGR (0.9 < area under the curve ≤1).
and were differentially expressed in vessels with and without stenosis after vascular transplantation, suggesting that these genes or their encoded proteins may be involved in the occurrence of VGR.
血管移植后出现的血脂异常似乎是导致静脉移植物再狭窄（VGR）的原因，严重影响了患者的预后和长期生活质量。
本研究分析了冠心病患者血管移植后再狭窄（VGR 组）和非狭窄（对照组）血管的遗传数据，鉴定可能导致其发生的关键基因。从基因表达综合数据库（GEO）中下载 GSE110398 数据集。使用 R 语言对 GSE110398 数据集进行了可重复性测试，包括差异表达基因（DEG）的鉴定、Metascape 软件的富集分析、通路富集分析以及蛋白质-蛋白质相互作用网络和枢纽基因网络的构建。
在 VGR 组和对照组之间鉴定出 24 个 DEG。鉴定出 4 个最重要的枢纽基因（、、、和），Pearson 相关系数显示和与 VGR 显著相关。也能敏感地预测 VGR（0.9<曲线下面积≤1）。
血管移植后狭窄和非狭窄血管中存在差异表达的和，提示这些基因或其编码蛋白可能参与 VGR 的发生。
