Zovari Fatemeh, Parsian Hadi, Bijani Ali, Moslemnezhad Ameneh, Shirzad Atena
Research Committee, Babol University of Medical Sciences, Babol, Iran.
Department of Biochemistry, School of Sciences, Babol University of Medical Sciences, Babol, Iran.
Int J Dent. 2020 Nov 17;2020:8860467. doi: 10.1155/2020/8860467. eCollection 2020.
In menopause, reduction of estrogen hormone affects oxidative stress process in serum. Oxidative stress in saliva plays a significant role in the pathogenesis of oral diseases. The aim of this study was to investigate the total antioxidant capacity and lipid peroxidation in the serum and saliva of premenopausal and postmenopausal women.
In this case control study, 50 postmenopausal women (case group) and 48 premenopausal women (control group) were selected. The unstimulated whole saliva and serum of the postmenopausal and premenopausal women were collected. The total antioxidant capacity (TAC) of the saliva and serum was measured by ferric reducing antioxidant power (FRAP). Also, malondialdehyde (MDA) was measured by thiobarbituric acid reactive substance (TBARS) method for serum and saliva. Then, the obtained data were analyzed by SPSS 17, whereby Mann-Whitney test and Spearman's correlation test were used. < 0.05 was considered statistically significant.
The postmenopausal group had significantly lower mean serum TAC and higher mean serum MDA than the control group ( < 0 < 001 and < 0.01, respectively). The mean salivary TAC and MDA, however, did not differ significantly between the case and control group ( = 0.64 and = 0.08, respectively).
In postmenopausal women, with elevation of serum MDA and reduction of serum TAC, the extent of serum oxidative stress grows, but MDA and TAC levels of saliva do not change.
在更年期，雌激素水平降低会影响血清中的氧化应激过程。唾液中的氧化应激在口腔疾病的发病机制中起重要作用。本研究的目的是调查绝经前和绝经后女性血清和唾液中的总抗氧化能力和脂质过氧化情况。
在这项病例对照研究中，选取了50名绝经后女性（病例组）和48名绝经前女性（对照组）。收集绝经后和绝经前女性的非刺激性全唾液和血清。通过铁还原抗氧化能力（FRAP）测定唾液和血清的总抗氧化能力（TAC）。此外，采用硫代巴比妥酸反应物质（TBARS）法测定血清和唾液中的丙二醛（MDA）。然后，使用SPSS 17对所得数据进行分析，采用曼-惠特尼检验和斯皮尔曼相关性检验。P < 0.05被认为具有统计学意义。
绝经后组的血清TAC均值显著低于对照组，血清MDA均值显著高于对照组（分别为P < 0.001和P < 0.01）。然而，病例组和对照组的唾液TAC和MDA均值无显著差异（分别为P = 0.64和P = 0.08）。
在绝经后女性中，随着血清MDA升高和血清TAC降低，血清氧化应激程度增加，但唾液的MDA和TAC水平未发生变化。
