Department Key Laboratory of Colloid Interface and Chemical Thermodynamics, Institute of Chemistry, Chinese Academy of Sciences, Bei Yi Jie 2, Zhong Guan Cun, Beijing, 100190, China.
School of Chemistry and Chemical Engineering Institution, University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
Angew Chem Int Ed Engl. 2021 Apr 6;60(15):8130-8138. doi: 10.1002/anie.202014348. Epub 2021 Mar 1.
Studies reveal that malignant tumors feature uneven distributions of some key biomarkers across the entire tumorous region. Nevertheless, only very limited progress has been made towards non-invasive and quantitative detection of tumor-specific biomarkers in vivo, especially with clinically compatible imaging modalities. Reported here is an Fe O nanoparticle-based glutathione (GSH) responsive magnetic resonance imaging (MRI) probe that can form particle aggregates within tumors in vivo to give rise to strong GSH concentration dependent interlocked relaxivities. A quantitative correlation between the interlocked MRI signals and local GSH concentration was established, and further applied for mapping the heterogeneous distribution of GSH within an intracranial tumor (2.4 mm × 1.6 mm) in vivo. This methodology will offer a practical route for quantitatively mapping tumor-specific biomarkers in vivo with unlimited detection depth, which largely challenges optical-imaging-based approaches.
研究表明，恶性肿瘤在整个肿瘤区域内的一些关键生物标志物分布不均匀。然而，在体内无创和定量检测肿瘤特异性生物标志物方面，仅取得了非常有限的进展，特别是在与临床兼容的成像方式方面。本文报道了一种基于 FeO 纳米颗粒的谷胱甘肽（GSH）响应磁共振成像（MRI）探针，该探针可在体内肿瘤内形成颗粒聚集物，从而产生强烈的与 GSH 浓度相关的互锁弛豫率。建立了互锁 MRI 信号与局部 GSH 浓度之间的定量相关性，并进一步应用于在体绘制颅内肿瘤（2.4mm×1.6mm）内 GSH 的不均匀分布。这种方法将为体内定量绘制肿瘤特异性生物标志物提供一条实用途径，具有无限的检测深度，这在很大程度上挑战了基于光学成像的方法。
