Department of Chemistry, Biochemistry and Environmental Protection, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21000, Novi Sad, Serbia.
Department of Biology, Science Faculty, Selcuk University, Alaeddin Keykubat Yerleskesi, Selcuklu, 42130, Konya, Turkey.
Chem Biodivers. 2021 Feb;18(2):e2000828. doi: 10.1002/cbdv.202000828. Epub 2020 Dec 29.
The aim of this research work was to study the bioactivity potentials (antioxidant, anti-inflammatory, and enzyme inhibitory) of ethanol (EtOH), water (H O) and chloroform (CHCl ) extracts of G. applanatum, G. lucidum, G. pfeifferi and G. resinaceum as well as their mycochemical profile: the total content of phenolics (TP) and sugars (TS) and LC/MS/MS detection of phenolics. LC/MS/MS profile showed that p-hydroxybenzoic and protocatechuic acids were mostly found. The highest ABTS and DPPH activities were detected in polar G. applanatum extracts (159.84±0.59 mg TE/g d.w., IC =0.85±0.30 μg/mL, respectively), while G. resinaceum CHCl extract was the most potent in NO assay (IC =41.21±0.18-81.89±0.81 μg/mL). The highest TP and TS were generally determined in G. applanatum EtOH extracts. Enzyme inhibitory effects were determined in H O extracts. Generally, CHCl extracts showed the most powerful anti-inflammatory potential. These results suggest that analyzed species are a promising source of bioactive compounds and may be considered as candidates for new food supplements or drug formulations.
本研究工作旨在研究垂榕、灵芝、松杉灵芝和皱盖假芝的乙醇（EtOH）、水（H₂O）和氯仿（CHCl₃）提取物的生物活性潜力（抗氧化、抗炎和酶抑制）及其真菌化学特征：总酚含量（TP）和总糖含量（TS）以及 LC/MS/MS 检测酚类物质。LC/MS/MS 图谱显示，主要存在对羟基苯甲酸和原儿茶酸。ABTS 和 DPPH 活性最高的是极性垂榕提取物（159.84±0.59mg TE/g d.w.，IC =0.85±0.30μg/mL），而皱盖假芝的 CHCl₃提取物在 NO 测定中最有效（IC =41.21±0.18-81.89±0.81μg/mL）。一般来说，总酚和总糖含量在垂榕 EtOH 提取物中最高。酶抑制作用在 H₂O 提取物中测定。一般来说，CHCl₃提取物表现出最强的抗炎潜力。这些结果表明，所分析的物种是生物活性化合物的有前途的来源，可被视为新型食品补充剂或药物制剂的候选物。
