使用 L. 叶提取物的银纳米粒子的绿色合成及其对膀胱（5637）和乳腺癌（MCF-7）细胞系的细胞毒性活性评价。

Green Synthesis of Silver Nanoparticles Using Extract of L. Leaves and Evaluation of Cytotoxic Activity Towards Bladder (5637) and Breast Cancer (MCF-7) Cell Lines.

机构信息

Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt.

Agro-BioSciences Research Division, Mohammed VI Polytechnic University, Ben-Guerir 43150, Morocco.

出版信息

Int J Nanomedicine. 2020 Dec 4;15:9771-9781. doi: 10.2147/IJN.S269880. eCollection 2020.

INTRODUCTION

L. is a very important medicinal and industrial flowering aromatic plant.

METHODS

The present study deals with L. leaves extract (JOLE) as a reducing and capping agent for the synthesis of silver nanoparticles (AgNPs) by the green pathway. Phenolic profile of the extract was evaluated using HPLC-PDA/MS/MS technique. L. leaves extract silver nanoparticles (JOLE-AgNPs) were characterized by ultraviolet light (UV), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), zeta potential and X-ray diffraction (XRD). JOLE-AgNPs were examined for their cytotoxic activities by neutral red uptake assay (NRU) against bladder (5637) and breast cancer (MCF-7) cell lines.

RESULTS

HPLC-PDA/MS/MS tentatively identified 51 compounds of different chemical classes. UV spectra showed absorption peak at λmax = 363 nm. The biosynthesized AgNPs were predominantly spherical in shape with an average size of 9.22 nm by TEM. The face cubic center (fcc) nature of silver nanoparticles was proved by XRD diffractogram. JOLE-AgNPs exhibited high cytotoxic activity against 5637 and MCF-7 cell lines compared to the cytotoxic activities of JOLE with IC of 13.09 µg/mL and 9.3 µg/mL, respectively.

DISCUSSION

The silver nanoparticles formed by L. showed high cytotoxic activities against MCF-7 and 5637 cell lines and can be introduced as a new alternative cytotoxic medication.

简介

L. 是一种非常重要的药用和工业芳香花卉植物。

方法

本研究涉及 L. 叶提取物（JOLE）作为一种还原和封端剂，通过绿色途径合成银纳米粒子（AgNPs）。采用高效液相色谱-光电二极管阵列-质谱/质谱联用技术（HPLC-PDA/MS/MS）评估提取物的酚类谱。L. 叶提取物银纳米粒子（JOLE-AgNPs）通过紫外光（UV）、傅里叶变换红外光谱（FTIR）、透射电子显微镜（TEM）、Zeta 电位和 X 射线衍射（XRD）进行表征。通过中性红摄取试验（NRU），JOLE-AgNPs 对膀胱癌（5637）和乳腺癌（MCF-7）细胞系的细胞毒性活性进行了检测。

结果

HPLC-PDA/MS/MS 技术初步鉴定了 51 种不同化学类别的化合物。紫外光谱显示在 λmax = 363nm 处有吸收峰。通过 TEM 观察到生物合成的 AgNPs 主要呈球形，平均粒径为 9.22nm。XRD 衍射图证明了银纳米粒子的面心立方（fcc）性质。与 JOLE 的细胞毒性相比，JOLE-AgNPs 对 5637 和 MCF-7 细胞系表现出更高的细胞毒性活性，IC 分别为 13.09µg/mL 和 9.3µg/mL。