University of Chinese Academy of Sciences, Beijing 100049, China.
National Engineering Laboratory for Industrial Enzymes, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
J Agric Food Chem. 2020 Dec 23;68(51):15249-15256. doi: 10.1021/acs.jafc.0c04851. Epub 2020 Dec 11.
Glucosylglycerol is a powerful osmolyte that has attracted attention as a useful moisturizing ingredient in the cosmetic industry. This study demonstrates two artificially designed synthetic routes for manufacturing glucosylglycerol by combining phosphorolysis and transglycosylation reactions. The overall Gibbs energy change of the synthetic routes was negative, indicating that they are thermodynamically favorable. biosystems were constructed through combining the phosphorolysis ability of sucrose/maltose phosphorylase and the transglycosylation capacity of glucosylglycerol phosphorylases from different organisms. A near-stoichiometric conversion of sucrose and glycerol with a high product yield of 98% was achieved under optimal reaction conditions. The large-scale glucosylglycerol production of this biosystem was investigated under a high concentration of substrates (2 mol/L sucrose and 2.4 mol/L glycerol), and the titer reached 1.78 mol/L (452 g/L) with a productivity of 24.3 g/L/h. To the best of our knowledge, this value presented the highest glucosylglycerol production level until now, which indicated a great industrial application potential for glucosylglycerol manufacturing.
葡糖基甘油是一种强效的渗透调节剂，作为化妆品行业中一种有用的保湿成分而受到关注。本研究通过结合磷酸解和转糖基反应，展示了两种人工设计的葡糖基甘油制造合成路线。合成路线的总吉布斯自由能变化为负值，表明它们在热力学上是有利的。通过结合蔗糖/麦芽糖磷酸化酶的磷酸解能力和来自不同生物体的葡糖基甘油磷酸化酶的转糖基能力，构建了生物系统。在最佳反应条件下，可近乎定量转化蔗糖和甘油，产物收率高达 98%。在高浓度底物（2 mol/L 蔗糖和 2.4 mol/L 甘油）下，对该生物系统进行了大规模葡糖基甘油生产研究，其浓度达到 1.78 mol/L（452 g/L），生产强度为 24.3 g/L/h。据我们所知，这一数值代表了迄今为止最高的葡糖基甘油生产水平，表明葡糖基甘油制造具有巨大的工业应用潜力。
