Lin Haixia, Tas Emir, Børsheim Elisabet, Mercer Kelly E
Arkansas Children's Nutrition Center, Little Rock, AR, USA.
Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA.
Diabetes Metab Syndr Obes. 2020 Dec 10;13:4929-4939. doi: 10.2147/DMSO.S273908. eCollection 2020.
MicroRNAs (miRNAs) are implicated in metabolic changes accompanying progression of obesity, insulin resistance (IR), and metabolic disorders in children. Identifying circulating miRNAs that uniquely associate with these disorders may be useful in early identification and prevention of obesity-related complications. We aimed to identify circulating miRNA signatures that distinguish adolescents with obesity and IR from those with obesity unaccompanied by IR.
Adolescents (aged 10-17 years) with obesity were recruited from a weight management clinic. Fasting serum samples were obtained from 33 participants. A total of 179 miRNAs were queried by a quantitative RT-PCR-based miRNA focus panel. Differentially expressed miRNAs were compared between groups using Student's -test or one-way ANOVA analysis, and the association between IR evaluated by homeostatic model assessment model (HOMA-IR > 4) and body mass index (BMI) status was assessed using Pearson's correlation analysis.
We found an expression pattern consisting of 12 elevated miRNAs linked to IR in obese adolescents. , , and were significantly correlated with clinical and biochemical markers of obesity and IR, suggestive of IR in adolescents at risk.
Specific signatures of circulating miRNAs reflected metabolic phenotypes and predicted the presence of IR in adolescents with obesity, suggesting that miRNA indicators may identify obesity-associated complications in childhood. Further studies will be needed to understand cause versus effect and the mechanisms by which IR status links to changes in blood miRNA profiles.
微小RNA（miRNA）与肥胖进展、胰岛素抵抗（IR）以及儿童代谢紊乱伴随的代谢变化有关。识别与这些疾病独特相关的循环miRNA可能有助于肥胖相关并发症的早期识别和预防。我们旨在识别区分肥胖伴IR青少年与不伴IR肥胖青少年的循环miRNA特征。
从体重管理诊所招募肥胖青少年（年龄10 - 17岁）。从33名参与者中获取空腹血清样本。通过基于定量RT-PCR的miRNA聚焦面板检测总共179种miRNA。使用学生t检验或单因素方差分析比较组间差异表达的miRNA，并使用Pearson相关分析评估通过稳态模型评估模型（HOMA-IR > 4）评估的IR与体重指数（BMI）状态之间的关联。
我们发现肥胖青少年中有12种与IR相关的miRNA表达模式升高。 、 和 与肥胖和IR的临床及生化标志物显著相关，提示有风险的青少年存在IR。
循环miRNA的特定特征反映了代谢表型，并预测了肥胖青少年中IR的存在，表明miRNA指标可能识别儿童期肥胖相关并发症。需要进一步研究以了解因果关系以及IR状态与血液miRNA谱变化相关的机制。
