Rodríguez-Sánchez Laura Katherine, Pérez-Bernal Jorge Enrique, Santamaría-Torres Mary Andrea, Marquínez-Casas Xavier, Cuca-Suárez Luis Enrique, Prieto-Rodríguez Juliet Angélica, Patiño-Ladino Oscar Javier
Research Group 'Química de Productos Naturales Vegetales Bioactivos QuiProNaB', Department of Chemistry, Faculty of Science, Universidad Nacional de Colombia, Bogotá, 11001, Colombia.
'Grupo de Investigación Fitoquímica Universidad Javeriana GIFUJ', Department of Chemistry, Faculty of Science, Pontificia Universidad Javeriana, Bogotá, 110231, Colombia.
Biotechnol Rep (Amst). 2020 Nov 25;28:e00559. doi: 10.1016/j.btre.2020.e00559. eCollection 2020 Dec.
Elicitation of cell suspensions culture is a strategy that could increase the production of secondary metabolites under controlled conditions. This research evaluated the effect of methyl jasmonate-MeJA and salicylic acid-SA as elicitors on the production of metabolites in cell suspensions of . The type of elicitor (MeJA or SA), the concentration of elicitor (10 μM and 100 μM), and time of exposition (3, 12, 24 h) on cell suspension were evaluated. Metabolic profiles of intracellular and extracellular extracts were analyzed by UHPLC-DAD and GC-MS. Differential production of metabolites was dependent on the type of elicitor, its concentration, and the time of exposition. Treatments with 100 μM SA were conducted to high production of 5-hydroxymethylfurfural (6.3 %), phenol (6.5 %), and (Z)-9-octadecenamide (8.8 %). This is the first report of elicitation on cell suspensions in the genus and contributes to understanding the effect of MeJA and SA on metabolite production in plant cell culture.
诱导细胞悬浮培养是一种可以在可控条件下提高次生代谢产物产量的策略。本研究评估了茉莉酸甲酯（MeJA）和水杨酸（SA）作为诱导剂对[植物名称]细胞悬浮培养物中代谢产物产量的影响。评估了诱导剂类型（MeJA或SA）、诱导剂浓度（10 μM和100 μM）以及细胞悬浮培养物的暴露时间（3、12、24小时）。通过超高效液相色谱-二极管阵列检测（UHPLC-DAD）和气相色谱-质谱联用（GC-MS）分析细胞内和细胞外提取物的代谢谱。代谢产物的差异产量取决于诱导剂类型、其浓度和暴露时间。用100 μM SA处理可使5-羟甲基糠醛（6.3%）、苯酚（6.5%）和（Z）-9-十八碳烯酰胺（8.8%）的产量较高。这是关于[植物属名]属细胞悬浮培养诱导的首次报道，有助于了解MeJA和SA对植物细胞培养中代谢产物产量的影响。
