Enshaei Hamidreza, Puiggalí-Jou Anna, Del Valle Luis J, Turon Pau, Saperas Núria, Alemán Carlos
Departament d'Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, C/ Eduard Maristany 10-14, Ed. I2, Barcelona, 08019, Spain.
Barcelona Research Center for Multiscale Science and Engineering, EEBE, Universitat Politècnica de Catalunya, C/ Eduard Maristany 10-14, Ed. C, Barcelona, 08019, Spain.
Adv Healthc Mater. 2021 Apr;10(7):e2001636. doi: 10.1002/adhm.202001636. Epub 2020 Dec 18.
Conducting polymers have been increasingly used as biologically interfacing electrodes for biomedical applications due to their excellent and fast electrochemical response, reversible doping-dedoping characteristics, high stability, easy processability, and biocompatibility. These advantageous properties can be used for the rapid detection and eradication of infections associated to bacterial growth since these are a tremendous burden for individual patients as well as the global healthcare system. Herein, a smart nanotheranostic electroresponsive platform, which consists of chloramphenicol (CAM)-loaded in poly(3,4-ethylendioxythiophene) nanoparticles (PEDOT/CAM NPs) for concurrent release of the antibiotic and real-time monitoring of bacterial growth is presented. PEDOT/CAM NPs, with an antibiotic loading content of 11.9 ± 1.3% w/w, are proved to inhibit the growth of Escherichia coli and Streptococcus sanguinis due to the antibiotic release by cyclic voltammetry. Furthermore, in situ monitoring of bacterial activity is achieved through the electrochemical detection of β-nicotinamide adenine dinucleotide, a redox active specie produced by the microbial metabolism that diffuse to the extracellular medium. According to these results, the proposed nanotheranostic platform has great potential for real-time monitoring of the response of bacteria to the released antibiotic, contributing to the evolution of the personalized medicine.
由于具有优异且快速的电化学响应、可逆的掺杂-去掺杂特性、高稳定性、易于加工性和生物相容性，导电聚合物已越来越多地用作生物医学应用中的生物接口电极。这些有利特性可用于快速检测和消除与细菌生长相关的感染，因为这些感染对个体患者以及全球医疗系统都是巨大的负担。在此，提出了一种智能纳米诊疗电响应平台，该平台由负载在聚（3,4-乙撑二氧噻吩）纳米颗粒（PEDOT/CAM NPs）中的氯霉素（CAM）组成，用于同时释放抗生素并实时监测细菌生长。通过循环伏安法证明，抗生素负载量为11.9±1.3% w/w的PEDOT/CAM NPs由于抗生素的释放而抑制了大肠杆菌和血链球菌的生长。此外，通过对β-烟酰胺腺嘌呤二核苷酸的电化学检测实现了对细菌活性的原位监测，β-烟酰胺腺嘌呤二核苷酸是一种由微生物代谢产生并扩散到细胞外介质中的氧化还原活性物质。根据这些结果，所提出的纳米诊疗平台在实时监测细菌对释放抗生素的反应方面具有巨大潜力，有助于个性化医学的发展。
