Malopolska Centre of Biotechnology, Jagiellonian University, 7a Gronostajowa St., 30-387 Kraków, Poland.
International Institute of Molecular and Cell Biology in Warsaw, 4 Ksiecia Trojdena St., 02-109 Warszawa, Poland.
Int J Mol Sci. 2020 Dec 16;21(24):9570. doi: 10.3390/ijms21249570.
Glycerol is an organic compound that can be utilized as an alternative source of carbon by various organisms. One of the ways to assimilate glycerol by the cell is the phosphorylative catabolic pathway in which its activation is catalyzed by glycerol kinase (GK) and glycerol-3-phosphate (G3P) is formed. To date, several GK crystal structures from bacteria, archaea, and unicellular eukaryotic parasites have been solved. Herein, we present a series of crystal structures of GK from (CtGK) in apo and glycerol-bound forms. In addition, we show the feasibility of an ADP-dependent glucokinase (ADPGK)-coupled enzymatic assay to measure the CtGK activity. New structures described in our work provide structural insights into the GK catalyzed reaction in the filamentous fungus and set the foundation for understanding the glycerol metabolism in eukaryotes.
甘油是一种有机化合物，可以被各种生物体用作碳的替代来源。细胞同化甘油的一种方法是磷酸化分解代谢途径，其中甘油激酶 (GK) 催化其激活，形成甘油-3-磷酸 (G3P)。迄今为止，已经从细菌、古菌和单细胞真核寄生虫中解决了几种 GK 晶体结构。在这里，我们展示了一系列来自 (CtGK) 的 GK 的晶体结构，分别为 apo 和甘油结合形式。此外，我们还展示了一种 ADP 依赖性葡萄糖激酶 (ADPGK) 偶联酶测定法来测量 CtGK 活性的可行性。我们工作中描述的新结构为丝状真菌中 GK 催化反应提供了结构见解，并为理解真核生物中的甘油代谢奠定了基础。
