School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia.
Vet Parasitol. 2012 Dec 21;190(3-4):578-82. doi: 10.1016/j.vetpar.2012.06.036. Epub 2012 Jul 3.
Current knowledge on the prevalence and genotypes of Cryptosporidium in fishes is still limited. This study investigated the prevalence of Cryptosporidium species in 171 ornamental fishes, belonging to 33 species, collected from 8 commercial aquariums around Perth, Western Australia. All samples were screened by nested PCR targeting the 18S rRNA locus. A total of 6 positives were identified by PCR at the 18S locus from 4 different species of fishes (red eye tetra, Moenkhausia sanctaefilomenae; gold gourami, Trichogaster trichopterus; neon tetra, Paracheirodon innesi; goldfish, Carassius auratus auratus), giving an overall prevalence of 3.5% (6/171). Four different genotypes were identified, only one of which has been previously reported in fish; piscine genotype 4 in a neon tetra isolate, a rat genotype III-like isolate in a goldfish, a novel genotype in three isolates from red eye (piscine genotype 7) which exhibited a 3.5% genetic distance from piscine genotype 1 and a piscine genotype 6-like from a gold gourami (1% genetic distance). Further biological and genetic characterisation is required to determine the relationship of these genotypes to established species and strains of Cryptosporidium.
目前，关于鱼类中隐孢子虫的流行情况和基因型的知识仍然有限。本研究调查了来自西澳大利亚珀斯 8 家商业水族馆的 33 个不同种类的 171 种观赏鱼中隐孢子虫的流行情况。所有样本均通过针对 18S rRNA 基因座的巢式 PCR 进行筛选。在 4 种不同鱼类（红眼半线脂鲤、莫纳亨萨塔菲洛梅纳；黄金斗鱼、三棘斗鱼；霓虹脂鲤、琴尾鱼；金鱼）的 18S 基因座上，通过 PCR 共鉴定出 6 个阳性，总流行率为 3.5%（6/171）。鉴定出了 4 种不同的基因型，其中只有一种以前在鱼类中报道过；霓虹脂鲤分离株的鱼类基因型 4、金鱼分离株的大鼠基因型 III 样株、3 个红眼分离株的新型基因型（鱼类基因型 7）与鱼类基因型 1 的遗传距离为 3.5%，而从黄金斗鱼分离株的鱼类基因型 6 样株的遗传距离为 1%。需要进一步进行生物学和遗传学特征分析，以确定这些基因型与已建立的隐孢子虫种和菌株的关系。
