Garcia-Aloy Mar, Groff Nelli, Masuero Domenico, Nisi Mauro, Franco Antonio, Battelini Furio, Vrhovsek Urska, Mattivi Fulvio
Metabolomics Unit, Research and Innovation Centre Fondazione Edmund Mach, Food Quality and Nutrition Department, 38098 San Michele all'Adige, Italy.
Agraria Riva del Garda S.C.A., 38066 Riva del Garda, Italy.
Metabolites. 2020 Dec 19;10(12):516. doi: 10.3390/metabo10120516.
The market of olive-based dietary supplements (OBDS) is composed of a broad range of natural extracts claiming different health effects and often sold without a clear statement on their chemical composition. The aim of this survey was to characterize the chemical profiles of 14 commercially available OBDS. As many as 378 compounds were tentatively annotated in the analyzed samples. Although for most of metabolites the annotation at level I was prevented due to the lack of the analytical standard, the spectra obtained from high-resolution tandem mass spectrometry (MS/MS) measurements were very informative, allowing annotation of dozens of metabolites at level II or III. A targeted method allowed the quantification of 26 selected compounds. A large qualitative and quantitative variability was observed. The products obtained from buds by glyceric maceration were those with the lowest concentrations of all the quantified elements. The dose of 5 mg of hydroxytyrosol, corresponding to the European Food Safety Authority (EFSA) health claim, was only reached by four products, all of them originating from the olive fruit or the leaves. If we also take into consideration oleuropein, two additional products provide this daily amount. This work demonstrates the high complexity and diversity in the composition of OBDS.
以橄榄为基础的膳食补充剂（OBDS）市场由多种声称具有不同健康功效的天然提取物组成，且通常在未明确说明其化学成分的情况下销售。本次调查的目的是对14种市售OBDS的化学特征进行表征。在所分析的样品中初步鉴定出多达378种化合物。尽管由于缺乏分析标准，大多数代谢物无法在一级水平进行注释，但从高分辨率串联质谱（MS/MS）测量获得的光谱信息非常丰富，能够在二级或三级水平注释数十种代谢物。一种靶向方法能够对26种选定化合物进行定量。观察到了较大的定性和定量变异性。通过甘油浸渍法从芽中获得的产品是所有定量元素浓度最低的产品。只有四种产品达到了欧洲食品安全局（EFSA）健康声明中规定的5毫克羟基酪醇剂量，所有这些产品均源自橄榄果实或叶子。如果我们还考虑橄榄苦苷，另外两种产品也能提供这一日剂量。这项工作证明了OBDS成分的高度复杂性和多样性。
